FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140957
Sequences flagged as poor quality0
Sequence length287
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT208940.9759187129867625No Hit
TTCTCTCCTCCCTCCTTCTCTTCTTTCCTTTTCCTTCCCTCCCTTCTCCT56990.266189372322751No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC31000.0277.827422
GATACGA31450.0276.979344
GGCGATA31450.0272.958681
TACGACA32050.0272.67086
GCGCTCC4200.0214.095252
CGCCCGC5150.0180.058268
TCCGCCC5000.0162.986
ACGACAG58250.0148.821467
CGCTCCG5650.0141.743363
CGGATCG200.007792282140.51
CGATACG67350.0133.51153
GACAGCA67100.0129.1939
GATACTA951.8189894E-12118.315794
CCGCCCG6750.0116.562967
CTATACT1950.0115.282063
CTCCGCC7500.0108.6533365
ATACGAC82950.0105.523215
ATACTAC1950.0100.87185
CGACAGC89150.097.8693168
CGACACT755.027887E-793.666678
Produced by FastQC (version 0.11.5)