FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n02_JRS_020_C9T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n02_JRS_020_C9T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739920
Sequences flagged as poor quality0
Sequence length287
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT52164370.49991891015245No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCATA72210.9759163152773273No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG66400.8973943128986919No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC45550.6156070926586658No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG41690.5634392907341335No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG40780.5511406638555519No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCTATGGGACAGCAGTATC38980.526813709590226No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT37320.5043788517677587No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCCT37010.5001892096442859No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG36520.49356687209428046No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT36050.487214834036112No Hit
GGCGATACGACAGCAGTATCTGCTATGGGCGACGCTATGGGCGAGCGCTA35090.47424045842793816No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT32260.435993080333009No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA32050.433154935668721No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA31410.4245053519299383No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC31240.4222078062493242No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA28860.39004216672072656No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC27800.37571629365336795No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC24420.33003567953292245No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC23400.31625040544923777No Hit
GGCGATACGACAGCAGTATCTATGGGAGAGCGCGCTCGTCGACTATGGGT21780.29435614661044435No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA20570.2780030273543086No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCTATGGGCGAGCGCTATG20550.2777327278624716No Hit
GGCGATACGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG20020.27056979132879233No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA18590.25124337766245003No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGAGAGCGCGCTCGTC18110.24475618985836306No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG17470.2361066061195805No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG17190.23232241323386313No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGGGAGAGCGCGCTCGTCGA14950.20204887014812412No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCATG14310.19339928640934156No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG14160.1913720402205644No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATA12980.17542437020218402No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCCTATG12440.16812628392258622No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGAGA12170.16447724078278733No Hit
GGCGATACGACAGCCTATGGGTCAGCAGTACTACTGACGATGGGACAGCA12130.16393664179911344No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT11980.16190939561033627No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGAGAGCGCGC11430.15447615958481997No Hit
GGCGATACGACAGCAGTATCTGCTATGGGAGAGCGCGCTCGTCGACTATG11220.15163801492053194No Hit
GGCGATACGACAGCAGTATCCTATGGGCGAGCGCTATGAGACAGCAGTAT10620.1435290301654233No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGGAGACTATGGGAGA10540.14244783219807547No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCTATGGGAGAGCATAGAC9410.12717591090928748No Hit
GGCGATACGACTATGGGAGAGCGCGCTCGTCGAGTATGGGACTACAGTCG9050.1223105200562223No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCATGGGCGAGCGCT8910.12041842361336362No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG8730.11798572818683101No Hit
GGCGATACGACAGCAGTATCTATTCAGTATTAATTCTATGGGCGAGCGCT8700.11758027894907558No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCATGACAGTCGACT8390.11339063682560277No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCCAT8050.10879554546437452No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG8030.10852524597253757No Hit
GGCGATACGACAGCAGTATCTGCTGCTATGGGTCAGCAGTACTACTGACG7770.10501135257865715No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGAGAGCGCGCTCGT7740.10460590334090171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTC257.1122486E-10281.07
CGATACG709700.0280.663453
GCGATAC710600.0280.5652
GGCGATA711450.0280.50631
GATACGA709800.0280.406164
TACGACA707850.0280.166356
ATACGAC711300.0279.755585
CGACAGC715200.0276.953168
GAATTCC401.1812517E-8175.624988
ACGACAG1289900.0153.592567
TTGGCCC100.0077920416140.49998280-281
CGATAGC402.4921083E-6140.499988
GATACGG752.5465852E-11131.133334
GACAGCA1555650.0127.029389
GCGATAT1900.0125.7105262
ACGGGGG301.3299359E-7117.08334280-281
ATACCTA1450.0116.275875
TTCGCGG550.0114.95454280-281
CGGTGGG3100.0113.30645280-281
GCGATCT2750.0112.42
Produced by FastQC (version 0.11.5)