FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n02_JRS_020_C9G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n02_JRS_020_C9G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808828
Sequences flagged as poor quality0
Sequence length287
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT56958170.42053440286439No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCATA64850.8017773865395362No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC55770.6895161888559743No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA55150.6818507766793435No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCTATGGGCGAGCGCTATG53180.6574945476665002No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG52740.6520545777346975No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCATG50150.6200329365452234No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT45400.5613059884178094No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCCT44930.5554951114452022No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG44010.544120628860524No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG42750.5285425331467258No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA41360.5113571735894406No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG37340.46165563012160804No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCTATGGGACAGCAGTATC36820.4552265747476596No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA34480.42629582556489143No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT30120.3723906689679388No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC29830.3688052342401598No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC28510.35248532444475217No Hit
GGCGATACGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG28320.3501362465196556No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG27270.3371545000914904No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC24480.3026601452966514No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG21430.2649512628148382No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT19810.2449222826113834No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG19730.24393319716923745No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA19480.24084230516253144No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGA18790.23231144322402292No Hit
GGCGATACGACAGCAGTATCTGCTATGGGCGACGCTATGGGCGAGCGCTA17700.21883515407478474No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGAGAG16570.20486432220447365No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC16510.2041225081228642No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG16090.1989298095515981No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA16090.1989298095515981No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC15490.1915116687355037No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCCAT14650.18112627159297157No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCATGGGCGAGCGCT12730.15738822098146948No Hit
GGCGATACGACAGCAGTATCTATGGGAGAGCGCGCTCGTCGACTATGGGT12350.15269006513127636No Hit
GGCGATACGACAGCAGCTATGGGTCAGCAGTACTATGGGAGAGCATAGAC11200.13847196190042876No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA10940.13525743421345454No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGGAGACTATGGGAGA10760.13303199196862622No Hit
GGCGATACGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACTATGGGA10200.12610839387360478No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGAGA9470.11708298921402326No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT8960.11077756952034301No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGAGAGCGCGC8850.10941757703739237No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG8780.10855212727551469No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGAG8670.10719213479256405No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAG8540.10558487094907694No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGAGAGCGCGCTCGT8480.1048430568674675No Hit
GGCGATACGACAGCAGTATCTGCTGTCTATGGGAGAGCGCGCTCGTCGAC8340.10311215734371214No Hit
GGCGATACGACAGCAGTATCTGCTATGGGAGAGCGCGCTCGTCGACTATG8300.10261761462263919No Hit
GGCGATACGACAGCAGTATCTGCTGCTATGGGCGAGCGCTATGAGCGACT8240.10187580054102975No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG8190.10125762213968854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATA771300.0280.799621
CGATACG769800.0280.616733
GCGATAC771350.0280.562842
GATACGA769250.0280.26944
TACGACA768550.0279.665476
ATACGAC771050.0279.651585
CGACAGC773200.0277.45668
ACGACAG1404050.0152.963447
GCGGCCT100.0077921073140.5280-281
GTCCCGT151.3947388E-4140.5280-281
GGTCTTC100.0077921073140.5280-281
GATACGC3550.0134.563394
GACAGCA1701450.0125.8385249
TCCTCCG501.8189894E-12112.4280-281
GGCGACA1001.8189894E-12112.41
ATACCTA2000.0112.45
CCTTGGA257.563116E-6112.4280-281
TCTCGGG2900.0106.586205280-281
CACCGGG1150.0103.847824280-281
GATACCT2200.0102.181824
Produced by FastQC (version 0.11.5)