FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n02_JRS_020_C9C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n02_JRS_020_C9C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756318
Sequences flagged as poor quality0
Sequence length287
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT56004974.04940778878726No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG63210.8357595614543089No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG59570.7876316575831859No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA52080.6885992400022213No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT50180.6634775319376242No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG45100.596310017743859No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC42340.5598174312921285No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC42170.5575696995179277No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG39100.5169783080661838No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG38930.514730576291983No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC32920.4352666470981783No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG29040.38396547483994825No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG22010.2910151550009388No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC21900.2895607403235147No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA20110.2658934469363416No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG19930.26351349564601134No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA19720.2607368858072927No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC17770.23495408016204825No Hit
GGCGATACGACACTATGGGACAGAGATATCATGCAGTATGGATCGACAAA16540.2186910796781248No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT16190.21406339661359375No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA15770.20851017693615648No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG15770.20851017693615648No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGACTATGGGCGAGCG15330.20269251822646028No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG15230.2013703230651657No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC15110.19978368887161221No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGAGA13600.17981854193606392No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG12900.1705631758070018No Hit
GGCGATACGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG12280.16236556580697536No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA12110.16011783403277458No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGACT11760.1554901509682435No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTACTATGGGCGAGCGC11570.1529779801617838No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT10530.1392271504843201No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT10300.13618610161334255No Hit
GGCGATACTATGGGAGAGCGCGCTCGTCGTCTATGGGAGAGCATAGACAT10280.13592166258108362No Hit
GGCGATACGACAGCTATGGGTCAGCAGTACTACTGACGATGGGACAGTCT9860.13036844290364635No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA9720.12851736967783395No Hit
GGCGATACGACAGCAGTATCTGCCTATGGGAGAGCGCGCTCGTCGACTAT9670.12785627209718664No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACG9600.1269307354842804No Hit
GGCGATACGACAGCAGTATCTGCTGTCTATGGGCGAGCGCTATGAGCGAC9310.12309636951652611No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGTCAGCAG8870.11727871080682993No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGTCAGCAGTACTAC8390.1109321740326159No Hit
GGCGATACGACAGCAGTACTATGGGCGAGCGCTATGAGCGACTATGGGAC8000.10577561290356702No Hit
GGCGATACGACAGCAGTATCTATGGGAGAGCGCGCTCGTCGACTATGGGT7910.10458563725840189No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGTC7740.1023379054842011No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCGATGGGACAGCAGT7690.1016768079035538No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA725100.0280.457464
CGATACG726950.0280.439483
ATACGAC725500.0280.26415
GGCGATA730050.0280.076231
GCGATAC729900.0279.768042
TACGACA725350.0279.682836
CGACAGC727350.0277.17538
CGACGGC451.09139364E-10187.333338
ACGACAG1339800.0150.483287
ATACGCT4150.0142.192785
GTGATAC1100.0140.52
GATACGC4900.0126.163274
TACGCTA4700.0125.5531856
GATGCGA1250.0123.644
CGATGCG1250.0123.643
GATACCT804.1836756E-11122.93754
GACAGCA1645300.0122.15729
GCGATCT702.3683242E-9120.4285662
TCTCGGG4350.0119.505745280-281
GCGATGC1350.0114.481482
Produced by FastQC (version 0.11.5)