FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n02_JRS_020_C9A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n02_JRS_020_C9A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043801
Sequences flagged as poor quality0
Sequence length287
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT71979368.95883410726758No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT85780.8218041561562022No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG81690.7826204420191205No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG75760.7258088467054544No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG74720.7158452616926023No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG60080.5755886418963001No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC57330.5492426238334702No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC51930.4975086247282767No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG48260.4623486660771546No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA47210.4522892773622558No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA45650.4373438998429778No Hit
GGCGATACGACACTATGGGACAGAGATATCATGCAGTATGGATCGACAAA42870.41071047067400784No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC41610.39863920421612936No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG41480.3973937560895228No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC30580.2929677208586694No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG29000.277830735935298No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA28860.27648948410664487No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT28470.2727531397268253No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGTATG24040.2303120997201574No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC22010.21086394820468654No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG20870.19994232617136792No Hit
GGCGATACGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG20770.19898428915090136No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG19480.18662561158688296No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGA18730.17944033393338385No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTGA17370.16641103045503886No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT16860.16152504165065945No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC16750.16047120092814626No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG16680.15980057501381967No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGAGAG16650.15951316390767972No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT16610.1591299490994931No Hit
GGCGATACGACAGCAGTATCTGCCTATGGGAGAGCGCGCTCGTCGACTAT16110.1543397639971604No Hit
GGCGATACGACTATGGGCGAGCGCTATGAGCGACTATGGGACAGCAGTAC16100.15424396029511372No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGACTATGGGCGAGCGCTATG15400.14753770115184792No Hit
GGCGATACGACAGCAGTATCTGCTGTCTATGGGAGAGCGCGCTCGTCGAC15380.1473460937477546No Hit
GGCGATACTATGGGCGAGCGCTATGAGCGACTATGGGACAGCAGTACTAT15370.14725029004570794No Hit
GGCGATACGACAGCAGTATCTGCTGCTATGGGCGAGCGCTATGAGCGACT14920.14293912345360849No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCAG14710.14092724571062876No Hit
GGCGATACGACAGCTATGGGTCAGCAGTACTACTGACGATGGGACAGTCT14320.13719090133080922No Hit
GGCGATACGACAGCAGTACTATGGGCGAGCGCTATGAGCGACTATGGGAC14220.13623286431034268No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA13760.13182589401619657No Hit
GGCGATACGCTATGGGAGAGCGCGCTCGTCGACTATGGGTCAGCAGTACT13700.13125107180391665No Hit
GGCGATACGACAGCAGGGAGAGCGCGCTCGTCGACTATGGGTCAGCAGTA13100.12550284968111738No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCTCG11740.11247354620277238No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG11560.11074907956593258No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCATCTATGGGAGAG11460.10979104254546604No Hit
GGCGATACGACAGCAGTATCTGCTATGGGCGAGCGCTATGAGCGACTATG11430.10950363143932608No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC10840.10385121301857347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATA973500.0280.249481
GCGATAC973600.0279.960972
CGATACG970050.0279.899233
ATACGAC967250.0279.88155
GATACGA969000.0279.332554
TACGACA966100.0279.283946
CGACAGC967000.0277.10618
CGATGCG600.0187.333343
ACGACAG1761200.0152.218987
GATACGT1700.0148.76474
CCAAGTT600.0128.79167280-281
ATACGTA1900.0125.7105265
GACAGCA2134350.0125.027139
GATACCT2550.0115.705884
CGACACT14650.0113.1672448
GTCCGGG257.563638E-6112.4280-281
TACGCTA5400.0111.879626
GATACGC6150.0107.3739854
ACGACAC16200.0104.074077
GGCGATC951.6734703E-10103.526311
Produced by FastQC (version 0.11.5)