FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n02_JRS_020_C8T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n02_JRS_020_C8T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451840
Sequences flagged as poor quality0
Sequence length287
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT22297449.3479992917847No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCTATGGGACAGCAGTATC80281.7767351274787533No Hit
GGCGATACGACAGCAGTATCTGCTATGGGCGACGCTATGGGCGAGCGCTA73851.6344281161473087No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT44670.9886242917847026No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC39930.8837199008498584No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCCT39360.8711048158640227No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG38070.8425548866855525No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCATA30310.6708126770538243No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC27820.6157046742209632No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT27820.6157046742209632No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT25070.5548424220963173No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG20830.461003895184136No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAG19810.4384295325779036No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC18390.407002478753541No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA18230.40346140226628896No Hit
GGCGATACGACAGCAGTATAGATATCATGCAGTATGGATCGACAAATGGC17470.38664128895184136No Hit
GGCGATACGACAGCAGTATCATGCAAGTATGGATCGACAAATGGCAGTAT17390.3848707507082153No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA17370.38442811614730876No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG16650.36849327195467424No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA16620.36782932011331443No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC15870.3512305240793201No Hit
GGCTGTTAATTTGTCTAGGAAAACTTATCAATTTTTGAAATTACCGCGTT13460.297893059490085No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG11550.25562145892351273No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA11160.2469900849858357No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCATG10710.2370308073654391No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGAGTATGGATC9750.21578434844192634No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC9570.21180063739376773No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG9510.21047273371104816No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG9030.1998495042492918No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCTATGGGCGAGCGCTATG9020.19962818696883855No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG8950.1980789660056657No Hit
GAGAAAGACGGCAAAATGGAATACAAAATACCAGGCCGACATCGACATTT8440.18679178470254956No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGAGA8180.18103753541076487No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG8060.1783817280453258No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCCAT7820.1730701133144476No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC7770.1719635269121813No Hit
GGCGATACGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACTATGGGA7440.1646600566572238No Hit
GGCGATACGACAGCAGTATCTGCTGTCATAGTATGGATCGACAAATGGCA7200.1593484419263456No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTCGA6720.14872521246458925No Hit
GGCGATCTATGGGACAGCAGTACTACTGACGACTATGGGACAGAGATATC6640.14695467422096317No Hit
CTATGGGACAGAGATATCATGCAAGTATGGATCGACAAATGGCAGTATTC6520.14429886685552407No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT6310.13965120396600567No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAT5720.12659348441926346No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACACTATGGGAGAGCGCG5510.12194582152974505No Hit
GGCGATACGACAGCAGTATCTGCTATGTGCGACGCTATGGGCGAGCGCTA5430.12017528328611898No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGA5430.12017528328611898No Hit
GGCGATACGACAGCAGTATCTGCTATGGGAGAGCGCGCTCGTCGACTATG5340.11818342776203966No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGAAGTATGGATCGACAAAT5270.11663420679886687No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACG5070.1122078611898017No Hit
GGCGATACGACAGCAGTATATGCTGTCATGGGACTGCACGACAGTCGACT5040.11154390934844191No Hit
GGCGATACGACAGCAGTATCTGCTATCATGGGACTGCACGACAGTCGACT4940.10933073654390935No Hit
GGCGATACTATTCGTACGATTCTTTGATTATGTAATAAAATGTGATCATT4910.10866678470254956No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG4820.10667492917847025No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGAACGACAGTCGACT4770.10556834277620397No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGAGAGCGC4730.10468307365439093No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA4720.10446175637393769No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATT4650.10291253541076487No Hit
GGCGATACGACTATGGGCGAGCGCTATGAGCGACTATGGGACAGCAGTAT4650.10291253541076487No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGAGCGAGCGCTATGAGCCT4650.10291253541076487No Hit
GGCGATACGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG4560.10092067988668556No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATA4540.10047804532577903No Hit
GGCGATACGACAGCAGTATCTGCTGCCTTTTAAGCATTTACCTTTTAAGA4540.10047804532577903No Hit
GGCGATACGACAGCAGTATCTGCTGTAATGGGACTGCACGACAGTCGACT4520.10003541076487252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG109.789903E-4280.999976
GCGATAC411700.0280.89762
GGCGATA413200.0280.7961
ATACGAC406600.0280.136145
CGATACG410700.0280.110573
TACGACA404600.0279.958226
GATACGA410000.0279.5954
CGACAGC404050.0276.792488
CGTGCAA700.0260.92866
GGATCGT2750.0260.563632
GATCGTG1800.0257.583343
GATACCT950.0251.421054
CGGATCG4000.0249.387511
GGCTGTT2000.0245.8751
GATCGTT351.8189894E-11240.857153
GCGATGC351.8189894E-11240.857152
CTATTCG700.0240.857158
CGTAATG950.0236.631587
TGCAACA1200.0234.166662
CGATACC1150.0232.130433
Produced by FastQC (version 0.11.5)