FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n02_JRS_020_C8G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n02_JRS_020_C8G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463013
Sequences flagged as poor quality0
Sequence length287
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT24638353.21297674147378No Hit
GGCGATACGACAGCAGTATCTGCTATGGGCGACGCTATGGGCGAGCGCTA66551.4373246539514009No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCATA46411.00234766626423No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCTATGGGACAGCAGTATC41470.8956551975862448No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG33480.7230898484491796No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC33180.7166105487318931No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG31660.6837820968309746No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCTATGGGCGAGCGCTATG28660.6189890996581089No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT27170.586808577728919No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC26600.5744979082660746No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCCT26530.5729860716653744No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT26290.5678026318915451No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG24310.5250392537574539No Hit
GGCTGTTAATTTGTCTAGGAAAACTTATCAATTTTTGAAATTACCGCGTT24290.5246073004429681No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG23620.5101368644076948No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA22150.4783882957929907No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA20400.4405923807754858No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCATG17810.38465442654957854No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT17630.3807668467192066No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC17620.3805508700619637No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG17370.3751514536308916No Hit
GGCGATACGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA17140.37018399051430523No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC16110.34793839481828803No Hit
GGCGATACGACAGCAGTATCATGCAAGTATGGATCGACAAATGGCAGTAT15520.3351957720409578No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCCAT12140.26219566189286264No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA11620.2509648757162326No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG10400.2246157235326006No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG9690.20928138086835574No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATA8930.19286715491789647No Hit
GGCGATACGACTATGGGCGAGCGCTATGAGCGACTATGGGACAGCAGTAT8410.18163636874126643No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG8400.18142039208402355No Hit
CTATGGGACAGAGATATCATGCAAGTATGGATCGACAAATGGCAGTATTC8370.1807724621122949No Hit
GGCGATACGACAGCAGTATAGATATCATGCAGTATGGATCGACAAATGGC7730.16694995604875026No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT7130.15399135661417715No Hit
GAGAAAGACGGCAAAATGGAATACAAAATACCAGGCCGACATCGACATTT6810.1470801035824048No Hit
GGCGATACGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG6770.14621619695343327No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGTCTATGGGAGAGCATGGGT6710.14492033700997597No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA6700.1447043603527331No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGAAATCGTCAGGCGAAAAT6580.14211264046581845No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC6530.14103275717960403No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGAGAGC6490.1401688505506325No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGATATGGGTCA6470.1397368972361467No Hit
TTGCAACATTTAGCCAGCAGCTAAAACAAAGTAAATAAGCGCAAGAGAAA6460.13952092057890383No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGGTATGG6240.13476943411956036No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG6190.13368955083334594No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAG6090.13152978426091708No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCATGGGCGAGCGCT5960.12872208771675955No Hit
GGCGATACGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACTATGGGA5630.12159485802774436No Hit
GGCGATACGACTATGGGCGAGCCTATGGGACAGCAGTATCATCTATGGGA5570.12029899808428705No Hit
GGCGATACGACAGCAGTATCTGCTATGAGCGACGCTATGGGCGAGCGCTA5410.11684337156840088No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGAGAGCG5130.1107960251656001No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACAGCACGACAGTCGACT5050.10906821190765702No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA4970.10734039864971393No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC4890.10561258539177086No Hit
GGCGATACGACAGCAGTATATGCTGTCATGGGACTGCACGACAGTCGACT4720.10194098221864181No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGA4710.10172500556139892No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTCGA4700.10150902890415604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG109.78998E-4281.01
CGTGCTA350.0281.06
GGCGATA426100.0280.802151
GCGATAC423700.0280.801062
ATACGAC418850.0280.496835
CGATACG422400.0280.3683
GATACGA421700.0280.300324
TACGACA416300.0280.223756
CGACAGC416250.0276.443248
GATCGTG2200.0255.454563
TCGTGCG450.0249.777775
GATACCT850.0247.941184
AGATACG1550.0244.741933
CGGATCG3350.0243.253721
CTGTTAA3600.0238.069443
ATCGTGC2150.0235.255814
CGGAACG302.1136657E-9234.166661
CGTAATG600.0234.166667
GGCTGTT3700.0231.635121
GGATCGT2950.0228.610152
Produced by FastQC (version 0.11.5)