FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n02_JRS_020_C8C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n02_JRS_020_C8C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661746
Sequences flagged as poor quality0
Sequence length287
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT31890048.19069552366014No Hit
GGCGATACGACAGCAGTATCTGCTGTCTAAAAAATATGAGTTCTGTGTGT169032.554303312751418No Hit
GGCGATACGACACTATGGGACAGAGATATCATGCAGTATGGATCGACAAA109141.6492732861248878No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG78681.1889758306057006No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG53900.8145119124256134No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC46720.7060110676906245No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG38990.5891988769104762No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG36530.5520244927812181No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC33250.5024586472755408No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT31870.48160472447132285No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG31010.4686088015643465No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA30470.46044857090182634No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC27740.4191940714413083No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT25420.38413530266899987No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC25350.38307749499052507No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGTATG22220.33577838022443657No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGACT22000.33245384180637283No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC21080.3185512266035609No Hit
GGCGATACGACAATGGGTCAGCAGTACTACTGACGATGGGACAGCAGTGT19380.2928616115548866No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG18790.28394580397917024No Hit
GGCGATACGACAGCAGTATCTGCTCGACTGATCGACAAATGGCAGTATTC18240.2756344579340109No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG18160.27442553487289684No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC16070.24284241990129143No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA15520.23453107385613212No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT15490.23407772770821433No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG14530.21957065097484535No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG13520.20430799732828003No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA12820.1937299205435318No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGACTATGGGCGAGCG12280.18556968988101175No Hit
GGCGATACGACAGCAGTATCTGCTGCTATGGGCGAGCTATGGGACAGCAG11650.17604942077473834No Hit
GGCGATAAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT11580.17499161309626352No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA11390.17212042082611756No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATA10800.16320461325040123No Hit
GGCGATACGACAGCAGTATCTGCTATGGGAGAGCATAGACATCGAGTATG10790.16305349786776197No Hit
GGCGATACGACAGCAGTATCTGCTGTCTATGGGCGAGCGCTATGAGCGAC10770.16275126710248342No Hit
GGCGATACGTTTTTAAAATAACAAATCAATTGTTTTATAATATTCGTACG10450.1579155748580271No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC9620.14537299809896848No Hit
GGCGATACGACAGCAGTATCATTATGTAATAAAATGTGATCATTAGGAAG9550.14431519042049365No Hit
GGCGATACGACAGCAGTATCATCGATGCAGCAGTATCATGGGAGAGCATA9520.1438618442725759No Hit
GGCGATACGACAGCAGTATCATCTATGGGAGAGCGCGCTCGTCGACTATA9360.14144399815034772No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA9230.13947949817603733No Hit
GGCGATAACTATGGGCGAGCTATGGGACAGCAGTATCATCGATCGATGAT9090.13736388281908768No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG9090.13736388281908768No Hit
GGCGATACGACAAACAAATCAATTGTTTTATAATATTCGTACGATTCTTT8710.1316214982787958No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCATGG8710.1316214982787958No Hit
GGCGATGCAATCTAGATTGCCCTAGAGAATGAATCGTTTTTAAAATAACA8540.12905253677392836No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACGAGCGCTA8450.127692498330175No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG8200.12391461376419352No Hit
GGCGATACGACAGCAGTATCTGCTGCTATGGGACGTATGGATCGACAAAT8070.12195011378988313No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAG7920.11968338305029423No Hit
GGCACAGAGACATCATGCAGTATGGATCGACAAATGGCAGTATTCATCCA7860.11877669075445867No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGAAT7690.11620772924959123No Hit
GGCGATACGACACTATAGGACAGAGATATCATGCAGTATGGATCGACAAA7510.11348765236208454No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCAGTATGGATCGAC7060.10668746014331781No Hit
GGCGATACGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG6850.10351403710789336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCCC307.2759576E-12281.08
GGCGATA565200.0280.87571
GCGATAC559150.0280.748722
GATACGA553950.0280.11234
CGATACG558500.0279.792453
TACGACA548650.0279.46356
ATACGAC551600.0279.446235
CGACAGC535050.0275.616858
GCGATGC1650.0255.454542
ATACGGT1000.0252.900015
CGGATCG2150.0241.79071
CGATAAG5000.0241.663
CGACACT19850.0227.206548
GGCGATC1300.0226.961551
CGATGCG500.0224.83
ACGACAC20550.0222.201977
GATACGT1800.0218.555564
GATACGG1200.0210.754
TGCAATC1450.0203.482766
GGCGATG2150.0202.581391
Produced by FastQC (version 0.11.5)