FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n02_JRS_020_C8A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n02_JRS_020_C8A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649294
Sequences flagged as poor quality0
Sequence length287
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT34845853.66721392774305No Hit
GGCGATACGACAGCAGTATCTGCTGTCTAAAAAATATGAGTTCTGTGTGT130452.009105274344134No Hit
GGCGATACGACACTATGGGACAGAGATATCATGCAGTATGGATCGACAAA102481.5783296934824596No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG92721.4280125798174634No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT61840.952419089041328No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG56940.8769525053365655No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG44760.6893641401275847No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC37940.584326976685446No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACCTATGGGCGAGCGCTA35190.5419732817490998No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC34620.5331945158895661No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG30080.46327241588556184No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA29200.44971923350593107No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG28780.44325066918838No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGTATG27380.4216887881298765No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTTAAAT26200.4035152026662806No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT25560.3936583427538219No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA23410.3605454539854057No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA23360.35977538680474486No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC21850.33651935794878746No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG21340.3286646727060469No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC17790.27398990287912717No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG17430.2684454191783691No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT17370.2675213385615761No Hit
GGCGATACGACAGCAGTATCTGCTGCTATGGGCGAGCTATGGGACAGCAG17270.26598120420025445No Hit
GGCGATACGACAGCTATGGGCGAGCGCTATGAGCGACTATGGGACAGCAG16700.2572024383407208No Hit
GGCGATAACTATGGGCGAGCTATGGGACAGCAGTATCATCGATCGATGAT16560.2550462502348705No Hit
GGCGATACGACAGCAGTATCTGCTCGACTGATCGACAAATGGCAGTATTC16060.2473455784282621No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG14960.2304041004537236No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAG14650.22562968393362637No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATA13220.20360576256672633No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAT12260.18882047269803817No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC11410.17572933062680388No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC10400.1601739735774549No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC10370.1597119332690584No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCATGG10250.15786377203547236No Hit
GGCGATACGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG10220.1574017317270759No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGACTAT9950.1532433689515073No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA9550.14708283150622062No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACATGCACGA9530.14677480463395626No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGA9060.13953617313574437No Hit
GGCGATACGACAGCAGTATCTGCTATGGGCGAGCGCTATGAGCGACTATG8630.13291359538206113No Hit
GGCGATACGACAGCTATGGGTCAGCAGTACTACTGACGATGGGACAGTCT8240.12690707137290658No Hit
GGCGATACGACAGCAGTATCTGCTGTCCAAATGAGACGATGAATTGGATT7670.11812830551337299No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGA6920.10657729780346038No Hit
GGCGATACGACAGCAGGGAGAGCGCGCTCGTCGACTATGGGTCAGCAGTA6850.10549920375053519No Hit
GGCACAGAGACATCATGCAGTATGGATCGACAAATGGCAGTATTCATCCA6750.10395906938921351No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT6740.10380505595308134No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGAGAGCG6670.10272696190015618No Hit
GGCGATACGACAGCAGTAACTATGGGACAGAGAGTATGGATCGACAAATG6570.10118682753883448No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAG6530.10057077379430582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCC109.790887E-4281.09
GGCGATA596200.0280.905761
GCGATAC592850.0280.64452
ATACGAC584950.0280.183355
CGATACG591050.0279.811433
TACGACA584700.0279.798526
GATACGA588450.0279.519684
CGACAGC573400.0275.560338
GATAACT2950.0247.661034
ATAGTCC750.0243.533336
GATACCT2150.0241.79074
GATACGT1050.0240.857134
TGATGAC1050.0227.476185
CGACACT21900.0220.694088
TACGTGT450.0218.555566
CGATAAG1300.0216.153853
ACGACAC23250.0211.505397
GCTATCG600.0210.753
TTGCAAC355.316906E-9200.71431
CGAAAGC1100.0191.590918
Produced by FastQC (version 0.11.5)