FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140957
Sequences flagged as poor quality0
Sequence length28
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATGC601.43299985E-820.16666622
AGATCGG901.4551915E-1118.3333341
CGGTTCA1303.7271093E-912.69230813
GGTTCAG1303.7271093E-912.69230814
AGGAATG1356.53381E-912.22222222
AGCGGTT1401.1204975E-811.78571411
ATCGGAA1554.143658E-911.3548393
GATCGGA1503.1035597E-811.02
TCGGAAG1503.1035597E-811.04
CGGAAGA1608.0051905E-810.31255
Produced by FastQC (version 0.11.5)