FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n01_JRS_020_C9T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n01_JRS_020_C9T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739920
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGATGTCTTTGAGAAGAAATCTCTGA14200.1919126392042383No Hit
CCCTCTCGTACGTTACTAAAATACATAA11020.1489350200021624No Hit
ACAGGGAAGATGCGGCTTGTTATATGTC10920.14758352254297763No Hit
ACCATTTTCAGCCCAACAACTACGGCAG10800.1459617255919559No Hit
TGGGCTGAGATGGATCCTCTAGGGCAAT9730.13150070277867879No Hit
TAAGTCGAGCCATTCGAACCCACCTGAG9320.1259595631960212No Hit
GGGAAGTGTCACAGAGAACCGGCAACTT8530.11528273326846146No Hit
GAACACTCACAACCTATATTTCAAAATT8280.11190398962049951No Hit
TGGGCGTTCTAGACATTTACTTAAATAT7770.10501135257865715No Hit
GTTGAACTCGCCTAAGTACCATGAGATT7750.10474105308682019No Hit
CCACCATGTAGGTCAGAAATAACAGAAA7740.10460590334090171No Hit
GATGACTAGTTGCAAGCTAGACAATTTC7660.1035247053735539No Hit
AACTTCTTCACGACTGTTAACCGAGTCT7640.10325440588171694No Hit
TGGTGATTCTCTTCGATATAGGGCTCTG7590.10257865715212455No Hit
GGTAATCTCCACCAGAGAGATCTCCTAG7550.10203805816845066No Hit
GTGCAGCTCAGTTGATAGGATTCTCGAT7490.10122715969293977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATG403.6124056E-622.0000027
GGATGGG403.6124056E-622.00000219
TTATGCT403.6124056E-622.0000022
TAGCGAT800.022.0000027
CGACCGT403.6124056E-622.00000212
CGCTGAC403.6124056E-622.00000210
GACCCCT403.6124056E-622.00000213
ACGGGCC656.730261E-1122.00000216
GGCCCCG1300.022.00000218
AGAACAC403.6124056E-622.00000216
GCCGCGG403.6124056E-622.00000219
CCCGTGG403.6124056E-622.0000025
ACTAGCG800.022.0000025
TAATTGC403.6124056E-622.0000029
CGGGCCA656.730261E-1122.00000217
CGCAGCG656.730261E-1122.0000026
GTATCCG656.730261E-1122.0000025
GGGCAAC403.6124056E-622.0000025
CCGGTCT403.6124056E-622.0000025
CCGCGGG403.6124056E-622.00000220
Produced by FastQC (version 0.11.5)