FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n01_JRS_020_C9G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n01_JRS_020_C9G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808828
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGATGTCCGGTATGAGCTGTAGGTTC12150.15021735152591156No Hit
AGAGCCCTCCCTCGTGGTGCTACCCCCT10560.1305592783632614No Hit
TATTGCTAGGGTCTGGTATAACATCCCA10370.12821020043816486No Hit
CAGGTATAGATTGTGGCACGTATCACCA9610.11881388873777861No Hit
AACAAAGCAAACGCGATTTCCTTACTCA9090.11238483336383015No Hit
TGGGCGTTCACCAGACCATCACTATTCA8500.10509032822800397No Hit
CGAAGGACAAATCAAGTCCGTACGCCAA8380.1036067000647851No Hit
TAACACGAGTTTCGATGGATGAGTACAA8140.10063944373834732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTG1100.022.0000024
CCAGGGT555.2477844E-922.0000026
GGGAGGT555.2477844E-922.00000211
ACGGGCC555.2477844E-922.0000022
CGGGCCA555.2477844E-922.0000023
GGCGCAG555.2477844E-922.0000024
AAACTAG250.00257970622.019
CAGCGGG250.00257970622.019
CGGAACT403.613095E-622.012
CCCCTGG250.00257970622.014
TCCGCCG850.022.08
GGTAAGA302.8716394E-422.04
GGTCGGA250.00257970622.07
TTAGCGA403.613095E-622.020
GCGGTAC302.8716394E-422.019
CCTAGCT250.00257970622.020
CGTGCCC850.022.018
AGCCCGT800.022.03
CGGTTGC1000.022.09
TAGAACA250.00257970622.014
Produced by FastQC (version 0.11.5)