FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n01_JRS_020_C9C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n01_JRS_020_C9C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756318
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCACAGTACACAGTAACAGGGTCTT11600.15337463871017218No Hit
AACGGGATCCCGATAGTGCATGCGTGAC9420.12455078419395017No Hit
GTGATGTGTGTCTCTCATGCACTTATCT9400.12428634516169126No Hit
GTCGTAAGTTCCGGCAGTTTGTAAACCT8410.11119661306487484No Hit
GAGAAATTCCTACTACCTCGCGCTCAGC8380.11079995451648646No Hit
TCAGCCTTCGTGGGTTTGCTGGTGTTTC8320.11000663741970969No Hit
TGTTCCGGTTGGGCAATCCTAACCATCG8310.10987441790358025No Hit
TTTACCACAAGACGTGCGCCGATTAGAA8040.10630449096808486No Hit
GAAGCGACAGCTCTCTACGCGTCCATAT7860.1039245396777546No Hit
CGCATGGGTGGATTCTGGTCGTAGATTA7850.10379232016162514No Hit
CTTAATCACCAGTTACGTAGTTTCATTT7660.10128014935516542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGCT403.612582E-622.021
GCCCCCC353.2133004E-522.020
CTAGATG403.612582E-622.06
CATGGGC454.0776285E-722.08
GAAATAG353.2133004E-522.09
GGGGAGA353.2133004E-522.014
CCCCTTG250.00257955322.016
AGCCGGT403.612582E-622.03
GGCAGGA750.022.010
AGTCCCC403.612582E-622.015
GAAGCGC353.2133004E-522.02
CTCGTGG302.871401E-422.021
GGTGTGC504.6182322E-822.05
TGCTTTA250.00257955322.017
GGTGTAC353.2133004E-522.018
ATAGGCA353.2133004E-522.011
CAACGGG403.612582E-622.08
GGATGCC250.00257955322.015
CGCGGAC454.0776285E-722.020
CCTAGCT302.871401E-422.05
Produced by FastQC (version 0.11.5)