FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n01_JRS_020_C9A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n01_JRS_020_C9A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043801
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGAGCAGACTAAAACTTCGCATGCT12600.12071266457878466No Hit
TTCACCGTCACTTCCAAATCATAATGAT11230.10758755739839299No Hit
CTCCACAAGAAACCCAGGAACTGCTGTA10930.10471344633699335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACA2900.022.0000021
GGGGCAC302.8724142E-422.014
TCGCCGG302.8724142E-422.011
GTGCAAC1400.022.05
CTATGCG302.8724142E-422.021
GGGGACC250.002580203622.020
GGCAGCG353.2148106E-522.015
AGCCGAT605.984475E-1022.08
GCCTTGG605.984475E-1022.09
CCCCTCC250.002580203622.021
GGAGCCC302.8724142E-422.015
CATATGG1050.022.018
GGGCTAG555.2514224E-922.03
TACCAGG250.002580203622.015
GGAGACG302.8724142E-422.011
CGCGGGG302.8724142E-422.016
CGCGGGC302.8724142E-422.020
CGCGGAC1100.022.017
CGACGAT605.984475E-1022.022
CGGCCTA403.6147612E-622.010
Produced by FastQC (version 0.11.5)