FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n01_JRS_020_C8T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n01_JRS_020_C8T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451840
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGCG403.6072506E-622.0000028
GCGCAGA403.6072506E-622.0000025
CCTTGAG403.6072506E-622.0000023
ACGCGAG605.9480953E-1022.03
GCGAGAG353.2096097E-522.05
GGTCGCG250.002577960522.06
GCGGTTC555.2314135E-922.010
GGGTAAG250.002577960522.03
AGGGTCC1000.022.01
CCTAAGA707.2759576E-1222.01
CCGAGAG353.2096097E-522.03
AGCTCGA353.2096097E-522.08
GGCTGCC250.002577960522.02
CCACGAC605.9480953E-1022.07
CCTGCGG250.002577960522.011
GAGGGGT353.2096097E-522.03
CCCGCAC302.868924E-422.04
GAAGGCG302.868924E-422.06
CCCGAGC353.2096097E-522.04
GAGGAGC6450.021.8294564
Produced by FastQC (version 0.11.5)