FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n01_JRS_020_C8G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n01_JRS_020_C8G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463013
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCCGC656.730261E-1122.0000026
GGCAGCC403.6075726E-622.05
GGAGCGG403.6075726E-622.06
GCGGTAC250.002578056222.05
GGCGTGC800.022.02
CCGTGAA302.869072E-422.06
CGTCTGG353.209832E-522.06
AGGGTAA353.209832E-522.08
TGACGCC504.6084097E-822.02
GCACCGT302.869072E-422.09
CCCGTAC250.002578056222.05
GAGCGGG403.6075726E-622.07
GGAGTGA250.002578056222.05
CCCCAGC454.070571E-722.05
AAGGGCA353.209832E-522.07
TGGGCTG454.070571E-722.01
GCGGAGT707.2759576E-1222.04
TAAGCTG302.869072E-422.06
GGCCTGA302.869072E-422.09
CCCATGC403.6075726E-622.06
Produced by FastQC (version 0.11.5)