FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n01_JRS_020_C8C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n01_JRS_020_C8C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661746
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGGA1600.022.0000025
CATGGGC555.2423275E-922.08
GGATGCG250.002579214322.09
TGCGGAG250.002579214322.03
AGCGGGT3450.022.08
AGACGCC900.022.08
GGAGTCC250.002579214322.06
GGACGCA250.002579214322.09
AAGGCGA555.2423275E-922.07
TAGCGGG3500.021.371437
CCGACGG850.020.7058819
TTACCGG850.020.7058812
CCCGAGG1550.020.5806474
AACCGAG701.8371793E-1020.4285723
CGTGAAC701.8371793E-1020.42857210
TGACTAG701.8371793E-1020.4285723
GAGTAGC3800.020.2631574
GGAATGG1850.020.2162171
GGGATCC601.4284524E-820.1666681
AAGAGAG1100.020.07
Produced by FastQC (version 0.11.5)