FastQCFastQC Report
Tue 6 Oct 2020
000000000-J3223_l01_n01_JRS_020_C8A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3223_l01_n01_JRS_020_C8A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649294
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGG504.6156856E-822.02
ACCGGAT504.6156856E-822.011
GCGACTC250.00257916322.013
CGCCTCG353.212397E-522.04
AATGCGG504.6156856E-822.010
GGCGCAA353.212397E-522.04
TCCCCCG250.00257916322.014
CCCGAGT504.6156856E-822.09
CAACTTG10000.021.238
AGTAGAC13950.021.2114725
TGAGTAG14200.021.1478863
GTGATCC9900.021.02
GAGTAGA14100.020.9858154
GGGCCCT1000.020.9000029
GGGCGTC1950.020.8717943
ATGGTGA1900.020.8421065
GGCGCAC900.020.7777794
TTGGGCG2600.020.7307681
TAGGTGC1700.020.7058837
CCGGACG801.8189894E-1220.6259
Produced by FastQC (version 0.11.5)