FastQCFastQC Report
Wed 27 Nov 2024
000000000-GNC2B_l01_n01_micro_run2_repeat_GyrBATH23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GNC2B_l01_n01_micro_run2_repeat_GyrBATH23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48344
Sequences flagged as poor quality0
Sequence length301
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCGGGGATGTACATCGGCGACACCTCCGACGGCACGGGCCTGCACCA1050.21719344696342877No Hit
AGGCCGGGCATGTACATCGGCGACACCTCCGACGGCACGGGCCTGCACCA660.13652159523415522No Hit
AGGCCGGGGATGTATATCGGCGACACCTCCGACGGCACGGGCCTGCACCA660.13652159523415522No Hit
CGGCCGGGGATGTACATCGGCGACACCTCCGACGGCACGGGCCTGCACCA650.1344530862154559No Hit
CGGCCGGGTATGTACATCGGCGACACCTCCGACGGCACGGGCCTGCACCA650.1344530862154559No Hit
AGACCGGGGATGTACATCGGCGACACCTCCGACGGCACGGGCCTGCACCA620.12824755915935793No Hit
AGGCCTGGGATGTACATCGGCGACACCTCCGACGGCACGGGCCTGCACCA620.12824755915935793No Hit
AGGCCCGGGATGTACATCGGCGACACCTCCGACGGCACGGGCCTGCACCA610.1261790501406586No Hit
AGTCCGGGGATGTACATCGGCGACACCTCCGACGGCACGGGCCTGCACCA590.12204203210325996No Hit
AGGCCAGGGATGTACATCGGCGACACCTCCGACGGCACGGGCCTGCACCA580.11997352308456065No Hit
AGGCCTGGGATGTATATCGGCGACACCTCCGACGGCACGGGCCTGCACCA580.11997352308456065No Hit
CGGCCTGGGATGTACATCGGCGACACCTCCGACGGCACGGGCCTGCACCA560.11583650504716202No Hit
AGGCCGGCGATGTACATCGGCGACACCTCCGACGGCACGGGCCTGCACCA560.11583650504716202No Hit
AGGCCGGGTATGTATATCGGCGACACCTCCGACGGCACGGGCCTGCACCA550.11376799602846269No Hit
CGGCCGGGGATGTATATCGGCGACACCTCCGACGGCACGGGCCTGCACCA510.1054939599536654No Hit
CGGCCTGGGATGTATATCGGCGACACCTCCGACGGCACGGGCCTGCACCA510.1054939599536654No Hit
CGGCCCGGGATGTATATCGGCGACACCTCCGACGGCACGGGCCTGCACCA490.10135694191626676No Hit
AGGCCCGGTATGTACATCGGCGACACCTCCGACGGCACGGGCCTGCACCA490.10135694191626676No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)