FastQCFastQC Report
Wed 13 Nov 2024
000000000-GNBP7_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GNBP7_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3939088
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC64396916.348175009037625Illumina Single End PCR Primer 1 (100% over 8bp)
TGAGGCAT1571413.9892736592835703No Hit
AGCACCAA1068502.712556815181585No Hit
GAGAGATT989752.5126374429817258No Hit
AGGAACTG890212.2599393565211034No Hit
CACGTCAG879672.2331818938800048No Hit
TGTAATGA877952.2288154009252903No Hit
TCACGGTT847482.151462470500786No Hit
TAAGATGG804292.041817801480952No Hit
TGCGTGAA789692.0047533845397716No Hit
TCCTTACG784531.9916539056756284No Hit
TGGCCATG776011.9700245335976245No Hit
GACAATGC673021.7085680746406275No Hit
ATGTCGTG660971.6779772373706807No Hit
AGTTCAGG649791.6495950331650373No Hit
ATTGACAT622171.5794772800201469RNA PCR Primer, Index 15 (100% over 8bp)
AGCCAACT618501.57016040261096No Hit
CATAGACC615821.563356797309428No Hit
CGGTTAAC578791.4693502658483384No Hit
CGCGGTTA574331.4580278480704163No Hit
GGAACTCC573951.4570631577664677RNA PCR Primer, Index 1 (100% over 8bp)
CGGAAGAA538961.3682354900423652No Hit
TGTAGGTG538321.3666107484778203No Hit
TACTAGGT489241.242013379746784No Hit
GCACTAAC458481.1639242383008452No Hit
ACGCGTGG451951.1473467970250981No Hit
CCTCAGAC431281.0948727218076875No Hit
TGTTAGCC427611.0855558443985003No Hit
TCGGTTCA408021.0358235205712591No Hit
GACCGCCA397701.0096245628429728No Hit
ATACGTCC368040.9343279459610956No Hit
ATAGCCTC355450.902366232996064No Hit
CTAGAGCT355390.902213913474388No Hit
TTGCCTGA339470.8617984670563338No Hit
TAGTGTAG338740.8599452462092748No Hit
AGCATTGG334420.8489782406485967No Hit
TGCGTACG330020.8378081423923507No Hit
GCAGAGAA327460.8313091761341712No Hit
CGTCGCTA301120.7644409061183706No Hit
ACGACGTG290230.7367949129341614No Hit
CCGTAACA280020.7108752076622813No Hit
ATATACAC274530.6969379714289196No Hit
GTGCTGAA264340.6710690393309314No Hit
AGACGGAA261240.6631991973776672TruSeq Adapter, Index 14 (100% over 8bp)
TCTCTGGA242440.615472413919161No Hit
GGCCATAA241780.6137968991807241No Hit
CTAGGAAC240490.6105220294646883No Hit
GCTCTAGT239730.6085926488567912No Hit
AGGTTGCA238160.604606954706267No Hit
TTACCGAC230940.5862778389312451No Hit
AGCTAGAG217100.5511428025979618No Hit
GACACTGA216030.5484264377947382No Hit
CTGACTTC182680.4637621703297819No Hit
TATCCTCT181770.4614519909176946No Hit
CTGTGCTA170240.43218125616894065No Hit
TCGTGGAA166120.42172198234718294No Hit
ACGCACAA159320.4044591032238935No Hit
CTCTCTAT154960.3933905513154314ABI Solid3 Adapter B (100% over 8bp)
AGTCCTTC153610.38996336207771953No Hit
ACTGCATA147150.3735636269105945No Hit
TCCAACAG146710.3724466170849699No Hit
GTAAGGAG144430.36665847526127876No Hit
GGCTACTC137760.3497256217682875No Hit
GTGATAGG136280.3459684069002774No Hit
TAGATCGC136180.3457145410308173No Hit
CACTGAGA133800.339672533337666No Hit
GTTGACGC129830.32959405832009847No Hit
GTTCGAGA125500.31860166617247443No Hit
CTTGAGGA123110.31253427189237715No Hit
GTCACAAC121050.30730463498149824No Hit
GTGAACCA120100.30489290922162693No Hit
AACTCTCG118660.30123724070140095TruSeq Adapter, Index 13 (100% over 8bp)
AAGGTACC115360.29285966700921634No Hit
ATGGATGC114620.2909810595752113No Hit
AGAGTAGA112220.28488827870816796No Hit
CTAGTATG104380.2649851945424931No Hit
TCTACACT102970.26140568578310514Illumina Single End PCR Primer 1 (100% over 8bp)
AAGGAGTA101990.25891780026239575ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
CTAAGCCT98270.24947398991847858No Hit
TTGTGAGC98000.24878855207093623No Hit
TGTCGAAC85170.2162175610192004No Hit
GTTAGCTC83120.21101331069526752No Hit
GATAGCGT58730.14909542513393964No Hit
AAGCAGCA49530.12573976514360685No Hit
ACGCGTGA42370.10756296889026089No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)