FastQCFastQC Report
Wed 13 Nov 2024
000000000-GNBP7_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GNBP7_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3939088
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGAGCG2065415.243371054416657No Hit
CGATCAGT1691534.294217341679089No Hit
CCTAAGAC1658254.209730780322754No Hit
TAGCGCTC1621174.1155973159269355No Hit
GCTACGCT1141572.898056605996109No Hit
ACTCGCTA978292.4835444143415937No Hit
ATCTCAGG969912.4622704544808345No Hit
CGAGGCTG929652.3600640554361823No Hit
CGGAGCCT870932.2109940168891886No Hit
CAGAGAGG870412.2096739143679955No Hit
ATGCGCAG868012.2035811335009527No Hit
AGCCATGC837642.1264820689459083No Hit
ACAGATTC788702.0022401124321165No Hit
CTCTCTAC760821.9314623080266298No Hit
AAGGACAC757361.9226785489433087No Hit
TACGCTGC755201.9171950461629697No Hit
GGAGCTAC746631.895438741150236No Hit
TCTTCACA734681.8651017697497492No Hit
AGCACCTC726641.8446909538451541No Hit
TTCACGCA696001.7669064514425674No Hit
TCCGTCTA686601.7430430597133144No Hit
GCTCATGA670111.7011805778393376No Hit
TGGCTTCA669321.6991750374706023No Hit
AATCCGTC637291.6178617994825197No Hit
ACACGACC608221.5440629912304575No Hit
TAGGCATG594291.50869947561466No Hit
TATCAGCA509611.2937258573558144No Hit
ATCATTCC509331.293015032921326No Hit
GGACTCCT487521.2376468867920698No Hit
TCCTGAGC424741.0782698939449944No Hit
AGGCAGAA422281.072024793556275No Hit
GCGTAGTA420761.068166032340481No Hit
CGTACTAG362830.9211015341622223No Hit
AAGAGGCA351270.8917546396526304No Hit
TAAGGCGA311820.7916045541506054No Hit
GATCTACG281840.7154955664864557No Hit
GTAACGAG251270.6378887701924912No Hit
GACATAGT242880.6165894237447855No Hit
ACGCTACT237420.602728347272262No Hit
TAGTCTCC215430.5469032425779775No Hit
TGAGTACG212240.538804921342199No Hit
ACTCACTG201910.5125805770269667No Hit
GTAGAGGA192280.4881332937979553No Hit
CGAGAGTT182870.46424451548175616TruSeq Adapter, Index 13 (100% over 8bp)
CTGCGTAG172010.4366746820583851No Hit
TGCTCGTA160050.40631232407095247No Hit
CGTAGCGA147240.37379210619310865No Hit
ACTACGAC138810.3523912133976189No Hit
CGAGCGAC137470.3489894107468531No Hit
GTCTGCTA137020.34784701433428244No Hit
CGAAGGAC130320.33083800108045314No Hit
AACGCTGA102890.261202593087537No Hit
GTCTATGA55990.14213950031073183No Hit
TTTTTTTT45680.11596592916939148No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)