FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9747
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC410.42064224889709656No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC380.3898635477582846No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC370.37960398071201396No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG370.37960398071201396No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC310.31804657843439005No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC300.3077870113881194No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC300.3077870113881194No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG290.29752744434184875No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC290.29752744434184875No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC270.2770083102493075No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC270.2770083102493075No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC260.2667487432030368No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG260.2667487432030368No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC260.2667487432030368No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC260.2667487432030368No Hit
TCCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG260.2667487432030368No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG250.2564891761567662No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG250.2564891761567662No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT250.2564891761567662No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT250.2564891761567662No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC250.2564891761567662No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT250.2564891761567662No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.2564891761567662No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC250.2564891761567662No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.2564891761567662No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT250.2564891761567662No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC250.2564891761567662No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT240.24622960911049552No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC240.24622960911049552No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT240.24622960911049552No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG240.24622960911049552No Hit
TTCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG240.24622960911049552No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC240.24622960911049552No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG230.2359700420642249No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG230.2359700420642249No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC230.2359700420642249No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT230.2359700420642249No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC230.2359700420642249No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT230.2359700420642249No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT230.2359700420642249No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT230.2359700420642249No Hit
TTCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.22571047501795424No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.22571047501795424No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG220.22571047501795424No Hit
TTCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.22571047501795424No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT220.22571047501795424No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT220.22571047501795424No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.21545090797168356No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG210.21545090797168356No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG210.21545090797168356No Hit
TCCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG210.21545090797168356No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.21545090797168356No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC200.20519134092541297No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC200.20519134092541297No Hit
TTCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG200.20519134092541297No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT200.20519134092541297No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT200.20519134092541297No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG200.20519134092541297No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT200.20519134092541297No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.1949317738791423No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC190.1949317738791423No Hit
TTCCGGCTGACCGTCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG190.1949317738791423No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT190.1949317738791423No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC190.1949317738791423No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.1949317738791423No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT190.1949317738791423No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT180.18467220683287164No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG180.18467220683287164No Hit
TCCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG180.18467220683287164No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC180.18467220683287164No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG180.18467220683287164No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.18467220683287164No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC180.18467220683287164No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG180.18467220683287164No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG180.18467220683287164No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.17441263978660101No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC170.17441263978660101No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG170.17441263978660101No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC170.17441263978660101No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.17441263978660101No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.17441263978660101No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.16415307274033036No Hit
TCCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.16415307274033036No Hit
TTCCGGCTGATCGTCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG160.16415307274033036No Hit
TCCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.16415307274033036No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT160.16415307274033036No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT160.16415307274033036No Hit
TTCCGGCTGACCGTCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG150.1538935056940597No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.1538935056940597No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC150.1538935056940597No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.1538935056940597No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT150.1538935056940597No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.1538935056940597No Hit
TCCCGGCTGACCGTCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG140.14363393864778906No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC140.14363393864778906No Hit
TCCCGGCCGACCGCCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG140.14363393864778906No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG140.14363393864778906No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT140.14363393864778906No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG130.1333743716015184No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG130.1333743716015184No Hit
TCCCGGCCGATCGCCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG130.1333743716015184No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT130.1333743716015184No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG130.1333743716015184No Hit
TCCCGGCTGACCGCCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG130.1333743716015184No Hit
TCCCGGCTGACCGCCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG120.12311480455524776No Hit
TTCCGGCTGATCGCCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG120.12311480455524776No Hit
TCCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.12311480455524776No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG120.12311480455524776No Hit
TCCCGGCTGACCGTCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG120.12311480455524776No Hit
TTCCGGCTGATCGCCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG120.12311480455524776No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG120.12311480455524776No Hit
TTCCGGCTGACCGCCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG110.11285523750897712No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG110.11285523750897712No Hit
TCCCGGCTGATCGTCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG110.11285523750897712No Hit
TCCCGGCTGATCGCCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG110.11285523750897712No Hit
TCCCGGCTGATCGCCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG110.11285523750897712No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG100.10259567046270648No Hit
TTCCGGCTGACCGCCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG100.10259567046270648No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC100.10259567046270648No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG100.10259567046270648No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT100.10259567046270648No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG100.10259567046270648No Hit
TCCCGGCCGATCGCCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG100.10259567046270648No Hit
TTCCGGCTGATCGTCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG100.10259567046270648No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG100.10259567046270648No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG100.10259567046270648No Hit
TCCCGGCTGATCGTCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG100.10259567046270648No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT100.10259567046270648No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG100.10259567046270648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)