FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14880
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA3262.1908602150537635No Hit
TTCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2982.002688172043011No Hit
TTCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2851.9153225806451613No Hit
TCCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2751.8481182795698925No Hit
TTCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA2701.8145161290322582No Hit
TTCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2621.760752688172043No Hit
TTCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA2551.7137096774193548No Hit
TCCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2541.706989247311828No Hit
TCCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA2491.6733870967741937No Hit
TTCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2451.646505376344086No Hit
TTCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA2441.6397849462365592No Hit
TCCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2441.6397849462365592No Hit
TCCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA2361.5860215053763442No Hit
TCCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA2341.5725806451612903No Hit
TTCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA2301.5456989247311828No Hit
TTCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA2151.4448924731182795No Hit
TCCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2111.418010752688172No Hit
TTCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2041.370967741935484No Hit
TCCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2021.35752688172043No Hit
TCCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2011.3508064516129032No Hit
TTCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1991.3373655913978495No Hit
TCCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1941.303763440860215No Hit
TTCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1911.2836021505376345No Hit
TCCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1911.2836021505376345No Hit
TCCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1891.2701612903225805No Hit
TCCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1891.2701612903225805No Hit
TTCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1871.256720430107527No Hit
TCCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1861.25No Hit
TTCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1861.25No Hit
TTCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1791.2029569892473118No Hit
TCCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1681.129032258064516No Hit
TCCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1651.1088709677419355No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC200.13440860215053765No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC200.13440860215053765No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT180.12096774193548387No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.12096774193548387No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC170.114247311827957No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.114247311827957No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT160.10752688172043011No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)