FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12166
Sequences flagged as poor quality0
Sequence length301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2652.178201545290153No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2512.0631267466710503No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA2442.005589347361499No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2141.7590004931777086No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2121.7425612362321221No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA2071.7014630938681572No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA2041.676804208449778No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2031.668584579976985No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2021.660364951504192No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1961.611047180667434No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1951.6028275521946407No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1931.5863882952490547No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1901.5617294098306758No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1861.5288508959395035No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1841.5124116389939175No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1751.4384349827387801No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1751.4384349827387801No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1701.3973368403748152No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1671.3726779549564359No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1641.348019069538057No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1641.348019069538057No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1641.348019069538057No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1631.3397994410652638No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1571.2904816702285056No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1531.2576031563373335No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1471.2082853855005753No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1431.1754068716094033No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1411.1589676146638173No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1411.1589676146638173No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1341.1014302153542659No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1341.1014302153542659No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1251.0274535590991287No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT280.23014959723820483No Hit
TCCCGGCTGATCGCCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG210.17261219792865362No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC200.16439256945586062No Hit
TTCCGGCTGACCGCCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG200.16439256945586062No Hit
TTCCGGCTGATCGCCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG200.16439256945586062No Hit
TTCCGGCTGATCGTCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG200.16439256945586062No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG190.15617294098306755No Hit
TCCCGGCTGACCGCCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG190.15617294098306755No Hit
TTCCGGCCGATCGTCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG190.15617294098306755No Hit
TCCCGGCTGATCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG190.15617294098306755No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT190.15617294098306755No Hit
TTCCGGCTGATCGTCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG190.15617294098306755No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.14795331251027452No Hit
TTCCGGCTGACCGTCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG170.1397336840374815No Hit
TTCCGGCTGACCGCCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG170.1397336840374815No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC170.1397336840374815No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.1397336840374815No Hit
TCCCGGCTGATCGTCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG170.1397336840374815No Hit
TCCCGGCTGATCGCCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG170.1397336840374815No Hit
TCCCGGCCGATCGCCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG170.1397336840374815No Hit
TTCCGGCTGATCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG170.1397336840374815No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.13151405556468848No Hit
TCCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.13151405556468848No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT160.13151405556468848No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT160.13151405556468848No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.13151405556468848No Hit
TCCCGGCTGACCGCCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG160.13151405556468848No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT160.13151405556468848No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT160.13151405556468848No Hit
TTCCGGCCGATCGTCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG160.13151405556468848No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT160.13151405556468848No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.12329442709189545No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG150.12329442709189545No Hit
TCCCGGCCGATCGTCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG150.12329442709189545No Hit
TTCCGGCTGATCGTCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG150.12329442709189545No Hit
TTCCGGCTGACCGTCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG150.12329442709189545No Hit
TCCCGGCTGACCGTCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG150.12329442709189545No Hit
TTCCGGCTGATCGCCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG150.12329442709189545No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.12329442709189545No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC150.12329442709189545No Hit
TCCCGGCTGATCGTCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG150.12329442709189545No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT150.12329442709189545No Hit
TCCCGGCTGACCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG140.11507479861910241No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC140.11507479861910241No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC140.11507479861910241No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC140.11507479861910241No Hit
TTCCGGCCGATCGTCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG140.11507479861910241No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.11507479861910241No Hit
TTCCGGCTGATCGCCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG140.11507479861910241No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.11507479861910241No Hit
TTCCGGCTGACCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG130.10685517014630938No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.10685517014630938No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT130.10685517014630938No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC130.10685517014630938No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.10685517014630938No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT130.10685517014630938No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT130.10685517014630938No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC130.10685517014630938No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG130.10685517014630938No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT130.10685517014630938No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)