FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13954
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC750.5374802923892791No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC670.4801490612010893No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC600.4299842339114232No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC590.4228178300128995No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC560.4013186183173284No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC540.3869858105202809No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT540.3869858105202809No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC540.3869858105202809No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC530.3798194066217572No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC520.3726530027232335No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT510.36548659882470974No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT500.35832019492618605No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC500.35832019492618605No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC490.3511537910276623No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC480.34398738712913857No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC480.34398738712913857No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT470.3368209832306149No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC460.3296545793320912No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC460.3296545793320912No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT450.32248817543356745No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT450.32248817543356745No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT440.3153217715350437No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC430.30815536763651996No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC420.3009889637379963No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT420.3009889637379963No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT410.2938225598394726No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC410.2938225598394726No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC410.2938225598394726No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT410.2938225598394726No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT400.28665615594094884No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC400.28665615594094884No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT400.28665615594094884No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC390.2794897520424251No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC380.2723233481439014No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC380.2723233481439014No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC380.2723233481439014No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT380.2723233481439014No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT370.26515694424537767No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT370.26515694424537767No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT360.2579905403468539No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC350.2508241364483302No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC350.2508241364483302No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT350.2508241364483302No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC350.2508241364483302No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC340.24365773254980652No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT340.24365773254980652No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT340.24365773254980652No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT340.24365773254980652No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC330.2364913286512828No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT330.2364913286512828No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.22932492475275906No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.22932492475275906No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT310.22215852085423535No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG310.22215852085423535No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC310.22215852085423535No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT310.22215852085423535No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC300.2149921169557116No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG300.2149921169557116No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG290.2078257130571879No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG290.2078257130571879No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT290.2078257130571879No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG280.2006593091586642No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG270.19349290526014046No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT270.19349290526014046No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG260.18632650136161674No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG260.18632650136161674No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG250.17916009746309303No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.17916009746309303No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT250.17916009746309303No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG240.17199369356456928No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT240.17199369356456928No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG240.17199369356456928No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG230.1648272896660456No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.15766088576752185No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG220.15766088576752185No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG220.15766088576752185No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG210.15049448186899814No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT210.15049448186899814No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG210.15049448186899814No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG210.15049448186899814No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG200.14332807797047442No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG200.14332807797047442No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG200.14332807797047442No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG190.1361616740719507No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.1361616740719507No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG180.12899527017342696No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG180.12899527017342696No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAATGGTGTGATGAACGCCGTG180.12899527017342696No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG180.12899527017342696No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.12182886627490326No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG170.12182886627490326No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.12182886627490326No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.12182886627490326No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAATGGTGTGATGAACGCCGTG170.12182886627490326No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAATGGTGTGATGAACGCCGTG170.12182886627490326No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG170.12182886627490326No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG170.12182886627490326No Hit
TCCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.12182886627490326No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAATGGTGTGATGAACGCCGTG170.12182886627490326No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG160.11466246237637953No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG160.11466246237637953No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAATGGTGTGATGAACGCCGTG160.11466246237637953No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.11466246237637953No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG160.11466246237637953No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.11466246237637953No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.11466246237637953No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAATGGTGTGATGAACGCCGTG160.11466246237637953No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG160.11466246237637953No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG160.11466246237637953No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG150.1074960584778558No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.1074960584778558No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG150.1074960584778558No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG150.1074960584778558No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.1074960584778558No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG150.1074960584778558No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAATGGTGTGATGAACGCCGTG150.1074960584778558No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.1003296545793321No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAATGGTGTGATGAACGCCGTG140.1003296545793321No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG140.1003296545793321No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC140.1003296545793321No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.1003296545793321No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.1003296545793321No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC140.1003296545793321No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG140.1003296545793321No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.1003296545793321No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)