FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16482
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG2031.2316466448246572No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1881.1406382720543622No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1781.0799660235408324No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1741.0556971241354205No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1560.9464870768110666No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1550.9404198519597137No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1540.9343526271083606No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1530.9282854022570076No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1520.9222181774056546No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1490.9040165028515956No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1470.8918820531488897No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1440.8736803785948308No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1390.8433442543380657No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1380.8372770294867127No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1380.8372770294867127No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1360.8251425797840068No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1350.8190753549326537No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1330.8069409052299479No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1320.8008736803785949No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1290.7826720058245359No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1280.7766047809731829No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1250.7584031064191239No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1250.7584031064191239No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1220.7402014318650649No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1210.7341342070137119No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1150.697730857905594No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1140.691663633054241No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1090.661327508797476No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG1080.6552602839461231No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG1060.6431258342434171No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG930.5642519111758282No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG920.5581846863244753No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC760.4611090887028273No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC660.4004368401892974No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC630.3822351656352384No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC630.3822351656352384No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC590.35796626622982647No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC580.3518990413784735No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT580.3518990413784735No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC530.32156291712170854No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC530.32156291712170854No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC520.31549569227035557No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC510.30942846741900254No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC500.30336124256764957No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC490.29729401771629654No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT470.28515956801359055No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC470.28515956801359055No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC460.27909234316223763No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC450.2730251183108846No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC450.2730251183108846No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC440.26695789345953164No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC440.26695789345953164No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC440.26695789345953164No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC430.2608906686081786No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC430.2608906686081786No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC430.2608906686081786No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT430.2608906686081786No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC420.25482344375682564No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC410.24875621890547264No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC410.24875621890547264No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC400.24268899405411964No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT380.23055454435141365No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC380.23055454435141365No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT370.22448731950006068No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT350.2123528697973547No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC340.2062856449460017No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT340.2062856449460017No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.2002184200946487No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.2002184200946487No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT330.2002184200946487No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT330.2002184200946487No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.2002184200946487No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT320.19415119524329572No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.19415119524329572No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.19415119524329572No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG310.18808397039194275No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT310.18808397039194275No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT310.18808397039194275No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT310.18808397039194275No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT300.18201674554058975No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC300.18201674554058975No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.17594952068923675No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG290.17594952068923675No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC270.16381507098653078No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC270.16381507098653078No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.16381507098653078No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG260.15774784613517778No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT260.15774784613517778No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT260.15774784613517778No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT260.15774784613517778No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC260.15774784613517778No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAATGGTGTGATGAACGCGGTG250.15168062128382478No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.15168062128382478No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT250.15168062128382478No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG230.13954617158111882No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT230.13954617158111882No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG230.13954617158111882No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT230.13954617158111882No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG220.13347894672976582No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT220.13347894672976582No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.13347894672976582No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAATGGTGTGATGAACGCGGTG210.12741172187841282No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG210.12741172187841282No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG210.12741172187841282No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC210.12741172187841282No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC210.12741172187841282No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAATGGTGTGATGAACGCGGTG210.12741172187841282No Hit
TCCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG200.12134449702705982No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG200.12134449702705982No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG200.12134449702705982No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTA200.12134449702705982No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG200.12134449702705982No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT200.12134449702705982No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC200.12134449702705982No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.11527727217570682No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.11527727217570682No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTA190.11527727217570682No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC190.11527727217570682No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG190.11527727217570682No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG180.10921004732435385No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG180.10921004732435385No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAATGGTGTGATGAACGCGGTG180.10921004732435385No Hit
TTCCGGCTGATCGCCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG180.10921004732435385No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT180.10921004732435385No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.10314282247300086No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCGGTA170.10314282247300086No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC170.10314282247300086No Hit
TCCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.10314282247300086No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC170.10314282247300086No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.10314282247300086No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)