FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12504
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG1591.2715930902111325No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG1541.2316058861164427No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG1501.199616122840691No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG1481.183621241202815No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG1371.0956493921944979No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG1331.063659628918746No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG1301.0396673064619322No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG1301.0396673064619322No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG1281.0236724248240563No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG1250.9996801023672425No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG1250.9996801023672425No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG1240.9916826615483045No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG1210.9676903390914907No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG1190.951695457453615No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG1160.9277031349968011No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG1140.9117082533589251No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG1130.9037108125399871No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG1090.8717210492642355No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG1080.8637236084452975No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG1060.8477287268074216No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG1030.8237364043506078No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG1030.8237364043506078No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG980.7837492002559182No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG970.7757517594369802No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG950.7597568777991043No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG930.7437619961612284No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAATGGCGTCATGAATGCCGTG920.7357645553422905No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG890.7117722328854766No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG850.6797824696097249No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG830.663787587971849No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG830.663787587971849No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAATGGCGTCATGAATGCCGTG830.663787587971849No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC400.3198976327575176No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC400.3198976327575176No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC390.31190019193857965No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC390.31190019193857965No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC360.28790786948176583No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC350.2799104286628279No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC340.27191298784388995No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC340.27191298784388995No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.263915547024952No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT320.2559181062060141No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC320.2559181062060141No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC310.24792066538707613No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC300.23992322456813817No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC300.23992322456813817No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT300.23992322456813817No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC290.23192578374920028No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.23192578374920028No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG290.23192578374920028No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.23192578374920028No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC280.22392834293026234No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC280.22392834293026234No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC280.22392834293026234No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC270.21593090211132437No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT270.21593090211132437No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC270.21593090211132437No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC260.20793346129238643No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC260.20793346129238643No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC260.20793346129238643No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC260.20793346129238643No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT260.20793346129238643No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC250.1999360204734485No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.1999360204734485No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.1999360204734485No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC240.19193857965451055No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT240.19193857965451055No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC240.19193857965451055No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT240.19193857965451055No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC230.18394113883557262No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.17594369801663468No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG220.17594369801663468No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG220.17594369801663468No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT220.17594369801663468No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.16794625719769674No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT210.16794625719769674No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG210.16794625719769674No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.16794625719769674No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT210.16794625719769674No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT200.1599488163787588No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG190.15195137555982086No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG190.15195137555982086No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.15195137555982086No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.15195137555982086No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT190.15195137555982086No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG190.15195137555982086No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCGGTG190.15195137555982086No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTC190.15195137555982086No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.15195137555982086No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.15195137555982086No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCGGTG190.15195137555982086No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC180.14395393474088292No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG180.14395393474088292No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCGGTG180.14395393474088292No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG180.14395393474088292No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG180.14395393474088292No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCGGTG170.13595649392194498No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.13595649392194498No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG170.13595649392194498No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.13595649392194498No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT170.13595649392194498No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT170.13595649392194498No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.12795905310300704No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT160.12795905310300704No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCGGTG160.12795905310300704No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG160.12795905310300704No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.12795905310300704No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.11996161228406908No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCGGTG150.11996161228406908No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCGGTG150.11996161228406908No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.11996161228406908No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.11996161228406908No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.11996161228406908No Hit
TCCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.11996161228406908No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT150.11996161228406908No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.11996161228406908No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG150.11996161228406908No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG140.11196417146513117No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.11196417146513117No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCGGTG140.11196417146513117No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT140.11196417146513117No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCGGTG140.11196417146513117No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG130.10396673064619322No Hit
TTCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.10396673064619322No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT130.10396673064619322No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG130.10396673064619322No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC130.10396673064619322No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.10396673064619322No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG130.10396673064619322No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG130.10396673064619322No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCGGTG130.10396673064619322No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCGGTG130.10396673064619322No Hit
TTCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG130.10396673064619322No Hit
TCCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG130.10396673064619322No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCGGTG130.10396673064619322No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT130.10396673064619322No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC130.10396673064619322No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)