FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11242
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1441.2809108699519658No Hit
TTCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1221.0852161537093044No Hit
TTCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1191.0585305105853051No Hit
TTCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1181.0496352962106386No Hit
TCCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1181.0496352962106386No Hit
TCCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1120.9962640099626401No Hit
TCCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1100.9784735812133072No Hit
TTCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1090.9695783668386408No Hit
TTCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1080.9606831524639745No Hit
TTCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1070.951787938089308No Hit
TTCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1070.951787938089308No Hit
TTCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1020.9073118662159757No Hit
TCCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1020.9073118662159757No Hit
TCCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA990.8806262230919765No Hit
TTCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA960.8539405799679772No Hit
TTCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA940.8361501512186443No Hit
TCCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA930.8272549368439779No Hit
TCCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA930.8272549368439779No Hit
TTCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA930.8272549368439779No Hit
TCCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA920.8183597224693115No Hit
TCCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA890.7916740793453123No Hit
TCCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA840.7471980074719801No Hit
TTCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA810.7205123643479807No Hit
TTCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA800.7116171499733144No Hit
TCCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA790.7027219355986479No Hit
TCCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA780.6938267212239815No Hit
TTCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA760.6760362924746487No Hit
TCCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA750.6671410780999822No Hit
TTCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA730.6493506493506493No Hit
TCCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA680.6048745774773172No Hit
TCCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA670.5959793631026508No Hit
TCCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA670.5959793631026508No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC580.5159224337306529No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT520.4625511474826543No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC480.4269702899839886No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC480.4269702899839886No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT470.41807507560932217No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT460.4091798612346558No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC450.4002846468599893No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC440.3913894324853229No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT440.3913894324853229No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC430.3824942181106565No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT430.3824942181106565No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC420.37359900373599003No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT410.3647037893613236No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT410.3647037893613236No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT410.3647037893613236No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT400.3558085749866572No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT400.3558085749866572No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT400.3558085749866572No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC400.3558085749866572No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC400.3558085749866572No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC390.34691336061199074No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT390.34691336061199074No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT390.34691336061199074No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT390.34691336061199074No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC380.33801814623732435No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC380.33801814623732435No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC370.3291229318626579No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT370.3291229318626579No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT370.3291229318626579No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC360.32022771748799145No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT350.311332503113325No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC350.311332503113325No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC350.311332503113325No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC340.3024372887386586No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT340.3024372887386586No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT340.3024372887386586No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT340.3024372887386586No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC330.29354207436399216No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.29354207436399216No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC330.29354207436399216No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.29354207436399216No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC330.29354207436399216No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.28464685998932576No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.28464685998932576No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.28464685998932576No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.28464685998932576No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.28464685998932576No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT310.2757516456146593No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT310.2757516456146593No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC300.26685643123999286No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC300.26685643123999286No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC300.26685643123999286No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG290.25796121686532647No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC290.25796121686532647No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.25796121686532647No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC290.25796121686532647No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC280.24906600249066002No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG280.24906600249066002No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC270.24017078811599363No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.24017078811599363No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC260.23127557374132715No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC260.23127557374132715No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG250.22238035936666076No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC250.22238035936666076No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC250.22238035936666076No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG240.2134851449919943No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT240.2134851449919943No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG230.2045899306173279No Hit
TCCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG230.2045899306173279No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.19569471624266144No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG200.1779042874933286No Hit
TTCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG200.1779042874933286No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG200.1779042874933286No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.1512186443693293No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.1512186443693293No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.1512186443693293No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.1512186443693293No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.1512186443693293No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.14232342999466288No Hit
TCCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.14232342999466288No Hit
TTCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.13342821561999643No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.13342821561999643No Hit
TTCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.13342821561999643No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.13342821561999643No Hit
TCCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.12453300124533001No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.12453300124533001No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.12453300124533001No Hit
TCCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.12453300124533001No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.12453300124533001No Hit
TCCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.11563778687066358No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG120.10674257249599715No Hit
TCCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.10674257249599715No Hit
TTCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG120.10674257249599715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)