FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10337
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2922.8248041017703396No Hit
TCCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2562.476540582373996No Hit
TTCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2352.2733868627261296No Hit
TTCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA2352.2733868627261296No Hit
TTCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2332.2540388894263326No Hit
TTCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2082.012189223178872No Hit
TCCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA2082.012189223178872No Hit
TTCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA2011.944471316629583No Hit
TTCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1971.9057753700299895No Hit
TTCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1871.8090355035310053No Hit
TCCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1861.7993615168811066No Hit
TCCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1861.7993615168811066No Hit
TTCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1841.7800135435813098No Hit
TCCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1791.7316436103318178No Hit
TTCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1771.712295637032021No Hit
TCCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1761.7026216503821225No Hit
TCCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1711.6542517171326303No Hit
TTCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1711.6542517171326303No Hit
TCCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1681.625229757182935No Hit
TCCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1681.625229757182935No Hit
TTCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1651.5962077972332396No Hit
TCCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1641.5865338105833415No Hit
TCCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1581.5284898906839508No Hit
TCCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1531.4801199574344586No Hit
TCCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1531.4801199574344586No Hit
TCCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1491.441424010834865No Hit
TTCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1431.3833800909354745No Hit
TTCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1371.325336171036084No Hit
TCCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1351.3059881977362873No Hit
TTCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1351.3059881977362873No Hit
TTCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1311.2672922511366935No Hit
TCCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1171.1318564380381155No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC270.26119763954725744No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC270.26119763954725744No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC240.23217567959756216No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC230.22250169294766373No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.21282770629776532No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT200.19347973299796847No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC200.19347973299796847No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC190.18380574634807004No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC190.18380574634807004No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.18380574634807004No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.17413175969817163No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC180.17413175969817163No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC180.17413175969817163No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC180.17413175969817163No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC180.17413175969817163No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC180.17413175969817163No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.17413175969817163No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.1644577730482732No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC170.1644577730482732No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT170.1644577730482732No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.1644577730482732No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC170.1644577730482732No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC160.15478378639837476No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.15478378639837476No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.15478378639837476No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT160.15478378639837476No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.15478378639837476No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.15478378639837476No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT160.15478378639837476No Hit
TTCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.14510979974847635No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.14510979974847635No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC150.14510979974847635No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.1354358130985779No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.1354358130985779No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC140.1354358130985779No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT140.1354358130985779No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC140.1354358130985779No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC130.1257618264486795No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT130.1257618264486795No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG130.1257618264486795No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT130.1257618264486795No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.1257618264486795No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC130.1257618264486795No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC130.1257618264486795No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC130.1257618264486795No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.11608783979878108No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC120.11608783979878108No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG120.11608783979878108No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG120.11608783979878108No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC120.11608783979878108No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC120.11608783979878108No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC110.10641385314888266No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC110.10641385314888266No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT110.10641385314888266No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG110.10641385314888266No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT110.10641385314888266No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT110.10641385314888266No Hit
TCCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG110.10641385314888266No Hit
TTCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG110.10641385314888266No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC110.10641385314888266No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT110.10641385314888266No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)