FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12028
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT510.42401064183571663No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT400.3325573661456601No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT390.3242434319920186No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT380.3159294978383771No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT380.3159294978383771No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT370.30761556368473564No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT370.30761556368473564No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT370.30761556368473564No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT370.30761556368473564No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT360.2993016295310941No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT360.2993016295310941No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT350.2909876953774526No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG330.2743598270701696No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.2743598270701696No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.2743598270701696No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT320.2660458929165281No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT320.2660458929165281No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG310.25773195876288657No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG290.2411040904556036No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT290.2411040904556036No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.2411040904556036No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.2411040904556036No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG280.2327901563019621No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT270.22447622214832058No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.22447622214832058No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG260.2161622879946791No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG260.2161622879946791No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT260.2161622879946791No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG250.20784835384103756No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG250.20784835384103756No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT250.20784835384103756No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTCATGAACGCTGTG240.19953441968739608No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG240.19953441968739608No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG240.19953441968739608No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG240.19953441968739608No Hit
TCCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG240.19953441968739608No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT240.19953441968739608No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT230.1912204855337546No Hit
TCCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG230.1912204855337546No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT230.1912204855337546No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG220.18290655138011308No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT220.18290655138011308No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT220.18290655138011308No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG210.17459261722647157No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG210.17459261722647157No Hit
TCCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG210.17459261722647157No Hit
TTCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG210.17459261722647157No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT210.17459261722647157No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTCATGAACGCTGTG210.17459261722647157No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG210.17459261722647157No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG210.17459261722647157No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTCATGAACGCTGTG210.17459261722647157No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT200.16627868307283006No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTCATGAACGCTGTG200.16627868307283006No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTCATGAACGCTGTG200.16627868307283006No Hit
TCCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.15796474891918855No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC190.15796474891918855No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.15796474891918855No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG190.15796474891918855No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.15796474891918855No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTCATGAACGCTGTG190.15796474891918855No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTCATGAACGCTGTG180.14965081476554706No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG180.14965081476554706No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC180.14965081476554706No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG180.14965081476554706No Hit
TTCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.14133688061190555No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG170.14133688061190555No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.14133688061190555No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG170.14133688061190555No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.14133688061190555No Hit
TTCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.13302294645826404No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.13302294645826404No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC160.13302294645826404No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG160.13302294645826404No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC160.13302294645826404No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC160.13302294645826404No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.13302294645826404No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG160.13302294645826404No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC150.12470901230462256No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG150.12470901230462256No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.12470901230462256No Hit
TCCCGGCTGATCGCCTGATTGCCAACGCCGTCATCCTGTGGATCGGCCTG150.12470901230462256No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG150.12470901230462256No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGTGTGATGAACGCCGTG150.12470901230462256No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG150.12470901230462256No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTCATGAACGCTGTG150.12470901230462256No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC150.12470901230462256No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.11639507815098105No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTCATGAACGCTGTG140.11639507815098105No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTCATGAACGCTGTG140.11639507815098105No Hit
TCCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.11639507815098105No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC140.11639507815098105No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.11639507815098105No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTCATGAACGCTGTG130.10808114399733955No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG130.10808114399733955No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC130.10808114399733955No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.10808114399733955No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC130.10808114399733955No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTCATGAACGCTGTG130.10808114399733955No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGTGTGATGAACGCCGTG130.10808114399733955No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)