FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11536
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG1140.9882108183079058No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG1060.9188626907073508No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG980.8495145631067961No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG980.8495145631067961No Hit
TTCCGGCTGACCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG940.8148404993065187No Hit
TTCCGGCTGATCGTCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG910.7888349514563107No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG900.7801664355062413No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG890.771497919556172No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG890.771497919556172No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG890.771497919556172No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG890.771497919556172No Hit
TTCCGGCTGATCGCCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG880.7628294036061026No Hit
TTCCGGCTGACCGCCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG870.7541608876560333No Hit
TCCCGGCTGACCGTCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG870.7541608876560333No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG850.7368238557558946No Hit
TCCCGGCTGACCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG830.7194868238557559No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG830.7194868238557559No Hit
TCCCGGCTGATCGCCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG820.7108183079056866No Hit
TTCCGGCTGATCGCCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG820.7108183079056866No Hit
TCCCGGCTGATCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG810.7021497919556172No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG800.6934812760055479No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG790.6848127600554785No Hit
TTCCGGCTGACCGCCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG780.6761442441054091No Hit
TTCCGGCTGACCGTCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG750.650138696255201No Hit
TCCCGGCTGATCGTCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG740.6414701803051317No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG740.6414701803051317No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG710.6154646324549237No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG700.6067961165048543No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG680.5894590846047156No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG680.5894590846047156No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG680.5894590846047156No Hit
TTCCGGCCGATCGTCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG680.5894590846047156No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG670.5807905686546463No Hit
TCCCGGCCGACCGCCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG660.5721220527045769No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG660.5721220527045769No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG660.5721220527045769No Hit
TTCCGGCCGATCGCCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG660.5721220527045769No Hit
TCCCGGCCGATCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG650.5634535367545076No Hit
TCCCGGCCGACCGCCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG640.5547850208044383No Hit
TTCCGGCCGACCGTCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG630.5461165048543689No Hit
TTCCGGCCGATCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG620.5374479889042996No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG620.5374479889042996No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG610.5287794729542302No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG610.5287794729542302No Hit
TCCCGGCTGACCGCCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG600.5201109570041609No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG600.5201109570041609No Hit
TTCCGGCCGATCGCCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG600.5201109570041609No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG590.5114424410540915No Hit
TCCCGGCCGATCGTCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG590.5114424410540915No Hit
TCCCGGCCGATCGCCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG580.5027739251040222No Hit
TTCCGGCCGACCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG570.4941054091539529No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG550.4767683772538141No Hit
TCCCGGCCGACCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG550.4767683772538141No Hit
TTCCGGCCGACCGCCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG540.46809986130374476No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG540.46809986130374476No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG530.4594313453536754No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT530.4594313453536754No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG530.4594313453536754No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG520.45076282940360607No Hit
TCCCGGCCGATCGCCTGATTGCCACCAAATCAGGATAGCGCCGTATCAGG520.45076282940360607No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC510.4420943134535367No Hit
TTCCGGCCGACCGCCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG500.4334257975034674No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT480.4160887656033287No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCAGTG480.4160887656033287No Hit
TCCCGGCTGACCGCCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG470.40742024965325935No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT460.39875173370319No Hit
TCCCGGCTGATCGCCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG460.39875173370319No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT440.3814147018030513No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC420.3640776699029126No Hit
TCCCGGCCGACCGTCTGATCGCCACCAAATCAGGATAGCGCCGTATCAGG420.3640776699029126No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT400.34674063800277394No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT390.33807212205270454No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC380.3294036061026352No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC380.3294036061026352No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT380.3294036061026352No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC370.32073509015256585No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT370.32073509015256585No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT370.32073509015256585No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC350.30339805825242716No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC350.30339805825242716No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT340.2947295423023578No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT340.2947295423023578No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC340.2947295423023578No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT330.28606102635228847No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.28606102635228847No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC320.27739251040221913No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT320.27739251040221913No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.27739251040221913No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC320.27739251040221913No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC320.27739251040221913No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT310.2687239944521498No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT310.2687239944521498No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT310.2687239944521498No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC300.26005547850208044No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT300.26005547850208044No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT300.26005547850208044No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC290.2513869625520111No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT290.2513869625520111No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC280.24271844660194172No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT280.24271844660194172No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT280.24271844660194172No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC280.24271844660194172No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT280.24271844660194172No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT280.24271844660194172No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT280.24271844660194172No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT270.23404993065187238No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC270.23404993065187238No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC260.22538141470180303No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT260.22538141470180303No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC250.2167128987517337No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.2167128987517337No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC240.20804438280166435No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC240.20804438280166435No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC230.199375866851595No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC230.199375866851595No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT220.19070735090152566No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.19070735090152566No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.1820388349514563No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.1820388349514563No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.1820388349514563No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC210.1820388349514563No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.1820388349514563No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC200.17337031900138697No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC190.1647018030513176No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC180.15603328710124825No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC170.1473647711511789No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC160.13869625520110956No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC150.13002773925104022No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG130.11269070735090152No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.11269070735090152No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.10402219140083217No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG120.10402219140083217No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG120.10402219140083217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)