FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17335
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC880.5076434958177098No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC840.4845687914623594No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT760.4384193827516585No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT730.42111335448514564No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC710.4095760023074704No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC700.4038073262186328No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC690.39803865012979517No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT660.3807326218632824No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC650.37496394577444475No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT630.36342659359676954No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT620.35765791750793197No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT610.35188924141909433No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT600.3461205653302567No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT590.3403518892414191No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC580.3345832131525815No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT580.3345832131525815No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT570.32881453706374386No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC560.3230458609749063No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT560.3230458609749063No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT550.31727718488606865No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT540.3115085087972311No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC530.30573983270839344No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC530.30573983270839344No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC520.2999711566195558No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC510.29420248053071824No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC510.29420248053071824No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC500.2884338044418806No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC500.2884338044418806No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC490.28266512835304297No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT490.28266512835304297No Hit
TTCCGGCTGATCGCCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA480.2768964522642054No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC480.2768964522642054No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT470.27112777617536776No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC470.27112777617536776No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT470.27112777617536776No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC470.27112777617536776No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC460.2653591000865302No Hit
TCCCGGCTGATCGCCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA460.2653591000865302No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC460.2653591000865302No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT460.2653591000865302No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT460.2653591000865302No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC450.25959042399769255No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT450.25959042399769255No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC450.25959042399769255No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT440.2538217479088549No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT430.24805307182001732No Hit
TTCCGGCCGATCGCCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA420.2422843957311797No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC420.2422843957311797No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT420.2422843957311797No Hit
TTCCGGCTGATCGTCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA420.2422843957311797No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC410.2365157196423421No Hit
TTCCGGCTGACCGTCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA410.2365157196423421No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT410.2365157196423421No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC410.2365157196423421No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT410.2365157196423421No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT410.2365157196423421No Hit
TCCCGGCTGACCGCCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA400.23074704355350445No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT400.23074704355350445No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT400.23074704355350445No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT390.22497836746466685No Hit
TTCCGGCTGATCGTCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA390.22497836746466685No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC390.22497836746466685No Hit
TCCCGGCTGATCGTCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA380.21920969137582924No Hit
TCCCGGCTGACCGTCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA380.21920969137582924No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT380.21920969137582924No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG370.2134410152869916No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT370.2134410152869916No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC370.2134410152869916No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC360.207672339198154No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG360.207672339198154No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC350.2019036631093164No Hit
TCCCGGCTGACCGTCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA330.1903663109316412No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG330.1903663109316412No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG310.17882895875396598No Hit
TCCCGGCTGACCGCCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA310.17882895875396598No Hit
TTCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG310.17882895875396598No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC300.17306028266512835No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG300.17306028266512835No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC290.16729160657629075No Hit
TTCCGGCCGACCGTCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA290.16729160657629075No Hit
TTCCGGCTGACCGTCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA290.16729160657629075No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.16729160657629075No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG280.16152293048745314No Hit
TTCCGGCTGATCGCCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA280.16152293048745314No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG280.16152293048745314No Hit
TTCCGGCTGACCGCCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA280.16152293048745314No Hit
TTCCGGCCGATCGTCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA280.16152293048745314No Hit
TCCCGGCCGACCGCCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA270.15575425439861554No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG270.15575425439861554No Hit
TTCCGGCCGACCGTCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA270.15575425439861554No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG270.15575425439861554No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG260.1499855783097779No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG260.1499855783097779No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG250.1442169022209403No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG250.1442169022209403No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG250.1442169022209403No Hit
TCCCGGCCGACCGTCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA250.1442169022209403No Hit
TCCCGGCCGATCGTCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA250.1442169022209403No Hit
TCCCGGCCGATCGCCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA240.1384482261321027No Hit
TTCCGGCTGACCGCCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA240.1384482261321027No Hit
TTCCGGCCGATCGCCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA240.1384482261321027No Hit
TTCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG240.1384482261321027No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT240.1384482261321027No Hit
TCCCGGCTGATCGTCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA230.1326795500432651No Hit
TTCCGGCCGACCGCCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA230.1326795500432651No Hit
TTCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.12691087395442746No Hit
TCCCGGCCGATCGCCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA220.12691087395442746No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.12691087395442746No Hit
TTCCGGCTGATCGCCTGATTGCCATTACTTCTATGCCTAGTGGAGGTGCC220.12691087395442746No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.12691087395442746No Hit
TCCCGGCTGATCGCCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA210.12114219786558986No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC210.12114219786558986No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG210.12114219786558986No Hit
TTCCGGCTGACCGTCTGATTGCCATTACTTCTATGCCTAGTGGAGGTGCC210.12114219786558986No Hit
TTCCGGCTGATCGTCTGATTGCCATTACTTCTATGCCTAGTGGTGGTGCC200.11537352177675222No Hit
TCCCGGCCGACCGCCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA200.11537352177675222No Hit
TCCCGGCCGACCGTCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA200.11537352177675222No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC190.10960484568791462No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.10960484568791462No Hit
TTCCGGCCGACCGCCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA190.10960484568791462No Hit
TTCCGGCTGATCGCCTGATTGCCATTACTTCTATGCCTAGTGGTGGTGCC180.103836169599077No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC180.103836169599077No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC180.103836169599077No Hit
TTCCGGCCGATCGTCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA180.103836169599077No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG180.103836169599077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)