FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9017
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT570.632139292447599No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT530.5877786403460131No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT520.5766884773206167No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT520.5766884773206167No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT510.5655983142952201No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT490.5434179882444272No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT480.5323278252190308No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT480.5323278252190308No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT480.5323278252190308No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT460.5101474991682378No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT450.4990573361428413No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT440.48796717311744486No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT430.4768770100920483No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT430.4768770100920483No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT430.4768770100920483No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT430.4768770100920483No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC390.4325163579904624No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT390.4325163579904624No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT370.4103360319396695No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT370.4103360319396695No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC360.39924586891427305No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT360.39924586891427305No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT360.39924586891427305No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT350.38815570588887655No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT330.36597537983808365No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT330.36597537983808365No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.35488521681268714No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT310.3437950537872907No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT300.3327048907618942No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT300.3327048907618942No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC290.32161472773649774No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.32161472773649774No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC290.32161472773649774No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC280.3105245647111013No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC280.3105245647111013No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT280.3105245647111013No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC270.2994344016857048No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC270.2994344016857048No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC260.28834423866030834No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT250.27725407563491183No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC250.27725407563491183No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC240.2661639126095154No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC230.2550737495841189No Hit
TTCCGGCTGATCGTCTGATTGCCAACCCGGGTTGCTACCCGACCACCATG230.2550737495841189No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC230.2550737495841189No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC230.2550737495841189No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.24398358655872243No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC220.24398358655872243No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC220.24398358655872243No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.23289342353332593No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.23289342353332593No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC210.23289342353332593No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.23289342353332593No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC200.22180326050792948No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC200.22180326050792948No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT190.21071309748253297No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC180.19962293445713652No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC180.19962293445713652No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC180.19962293445713652No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC170.18853277143174005No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC170.18853277143174005No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC150.1663524453809471No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC150.1663524453809471No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC150.1663524453809471No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC150.1663524453809471No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC140.15526228235555065No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC140.15526228235555065No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC140.15526228235555065No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.15526228235555065No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC140.15526228235555065No Hit
TCCCGGCCGACCGTCTGATTGCCAACCCGGGTTGCTACCCGACCACCATG130.14417211933015417No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC130.14417211933015417No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC120.1330819563047577No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG120.1330819563047577No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.1330819563047577No Hit
TTCCGGCTGACCGCCTGATCGCCAACCCGGGTTGCTACCCGACCACCATG120.1330819563047577No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC120.1330819563047577No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG110.12199179327936122No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG110.12199179327936122No Hit
TTCCGGCTGACCGTCTGATTGCCAACCCGGGTTGCTACCCGACCACCATG110.12199179327936122No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC110.12199179327936122No Hit
TTCCGGCTGATCGCCTGATTGCCAACCCGGGTTGCTACCCGACCACCATG110.12199179327936122No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC110.12199179327936122No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC110.12199179327936122No Hit
TCCCGGCTGATCGTCTGATTGCCAATCCAGGCTGCTATCCAACCACCATG110.12199179327936122No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG100.11090163025396474No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC100.11090163025396474No Hit
TCCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG100.11090163025396474No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC100.11090163025396474No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC100.11090163025396474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)