FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16962
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC890.5247022756750384No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC720.4244782454899187No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC700.41268718311519864No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT690.4067916519278387No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC680.4008961207404787No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT680.4008961207404787No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC670.3950005895531188No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT660.38910505836575876No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC660.38910505836575876No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC640.37731399599103876No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC630.3714184648036788No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC630.3714184648036788No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC620.3655229336163188No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT610.35962740242895885No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC610.35962740242895885No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC610.35962740242895885No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC590.34783634005423886No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC590.34783634005423886No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT590.34783634005423886No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC580.3419408088668789No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC570.3360452776795189No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT560.33014974649215895No Hit
TTCCGGCTGACCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG560.33014974649215895No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT550.324254215304799No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC540.31835868411743895No Hit
TCCCGGCTGACCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG540.31835868411743895No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT530.312463152930079No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC530.312463152930079No Hit
TTCCGGCTGATCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG530.312463152930079No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC520.306567621742719No Hit
TTCCGGCTGACCGCCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG520.306567621742719No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC520.306567621742719No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT510.30067209055535904No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC510.30067209055535904No Hit
TTCCGGCTGATCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG510.30067209055535904No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT500.29477655936799907No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT500.29477655936799907No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC490.28888102818063904No Hit
TCCCGGCTGACCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG480.2829854969932791No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT470.2770899658059191No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT470.2770899658059191No Hit
TTCCGGCTGACCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG460.27119443461855913No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC450.2652989034311991No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT450.2652989034311991No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT450.2652989034311991No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC450.2652989034311991No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC450.2652989034311991No Hit
TCCCGGCTGATCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG450.2652989034311991No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT440.25940337224383914No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT430.25350784105647917No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT420.24761230986911922No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT420.24761230986911922No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT420.24761230986911922No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC410.24171677868175925No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC410.24171677868175925No Hit
TTCCGGCTGACCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG410.24171677868175925No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT410.24171677868175925No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC400.23582124749439923No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT400.23582124749439923No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT390.22992571630703926No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC390.22992571630703926No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT390.22992571630703926No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT390.22992571630703926No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT390.22992571630703926No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC380.2240301851196793No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG380.2240301851196793No Hit
TTCCGGCCGATCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG380.2240301851196793No Hit
TTCCGGCTGATCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG380.2240301851196793No Hit
TTCCGGCCGATCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG380.2240301851196793No Hit
TTCCGGCTGATCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG380.2240301851196793No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC380.2240301851196793No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT380.2240301851196793No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT370.21813465393231932No Hit
TTCCGGCCGACCGCCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG370.21813465393231932No Hit
TCCCGGCTGATCGCCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG360.21223912274495935No Hit
TCCCGGCTGACCGCCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG350.20634359155759932No Hit
TCCCGGCTGATCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG350.20634359155759932No Hit
TTCCGGCCGATCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG350.20634359155759932No Hit
TCCCGGCCGACCGCCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG340.20044806037023935No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT340.20044806037023935No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT340.20044806037023935No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG330.19455252918287938No Hit
TCCCGGCTGATCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG330.19455252918287938No Hit
TTCCGGCCGACCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG330.19455252918287938No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.19455252918287938No Hit
TCCCGGCCGATCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG320.18865699799551938No Hit
TTCCGGCTGATCGCCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG320.18865699799551938No Hit
TCCCGGCCGATCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG320.18865699799551938No Hit
TCCCGGCTGATCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG320.18865699799551938No Hit
TTCCGGCCGATCGCCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG320.18865699799551938No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC310.1827614668081594No Hit
TTCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG310.1827614668081594No Hit
TTCCGGCCGACCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG310.1827614668081594No Hit
TCCCGGCCGATCGCCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG300.17686593562079944No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG290.17097040443343944No Hit
TCCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG290.17097040443343944No Hit
TCCCGGCCGACCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG290.17097040443343944No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.17097040443343944No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG280.16507487324607947No Hit
TTCCGGCCGACCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG280.16507487324607947No Hit
TCCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG280.16507487324607947No Hit
TCCCGGCCGATCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG280.16507487324607947No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG280.16507487324607947No Hit
TCCCGGCTGACCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG270.15917934205871948No Hit
TTCCGGCTGATCGCCTGATCGCCAATCCGGGTTGCTATCCGACCACCATG270.15917934205871948No Hit
TTCCGGCTGACCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG270.15917934205871948No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG270.15917934205871948No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG260.1532838108713595No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG260.1532838108713595No Hit
TCCCGGCCGACCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG260.1532838108713595No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG250.14738827968399953No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG250.14738827968399953No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG250.14738827968399953No Hit
TTCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG250.14738827968399953No Hit
TTCCGGCCGATCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG250.14738827968399953No Hit
TTCCGGCTGATCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG250.14738827968399953No Hit
TTCCGGCTGATCGCCTGATCGCCAATCCGGGCTGCTACCCGACCACCATG240.14149274849663954No Hit
TCCCGGCCGACCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG240.14149274849663954No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG240.14149274849663954No Hit
TCCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG240.14149274849663954No Hit
TTCCGGCTGATCGTCTGATTGCCAATCCGGGCTGCTACCCGACCACCATG240.14149274849663954No Hit
TTCCGGCCGACCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG230.13559721730927957No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG230.13559721730927957No Hit
TCCCGGCCGACCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG230.13559721730927957No Hit
TCCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG230.13559721730927957No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG230.13559721730927957No Hit
TCCCGGCTGATCGCCTGATTGCCAATCCGGGCTGCTACCCGACCACCATG230.13559721730927957No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG220.12970168612191957No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG220.12970168612191957No Hit
TTCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.12970168612191957No Hit
TTCCGGCTGACCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG220.12970168612191957No Hit
TTCCGGCTGATCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACCATG210.12380615493455961No Hit
TTCCGGCCGATCGCCTGATTGCCAATCCGGGCTGCTACCCGACCACCATG210.12380615493455961No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG210.12380615493455961No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG200.11791062374719961No Hit
TTCCGGCTGACCGCCTGATCGCCAATCCGGGTTGCTATCCGACCACCATG200.11791062374719961No Hit
TCCCGGCCGATCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG200.11791062374719961No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG200.11791062374719961No Hit
TTCCGGCCGATCGTCTGATTGCCAATCCGGGCTGCTACCCGACCACCATG190.11201509255983964No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.11201509255983964No Hit
TCCCGGCTGACCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG190.11201509255983964No Hit
TCCCGGCTGACCGTCTGATTGCCAATCCGGGCTGCTACCCGACCACCATG190.11201509255983964No Hit
TCCCGGCTGATCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACCATG190.11201509255983964No Hit
TCCCGGCTGACCGCCTGATCGCCAATCCGGGCTGCTACCCGACCACCATG180.10611956137247967No Hit
TTCCGGCCGATCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG180.10611956137247967No Hit
TCCCGGCTGACCGCCTGATTGCCAATCCGGGCTGCTACCCGACCACCATG180.10611956137247967No Hit
TTCCGGCTGACCGCCTGATTGCCAATCCGGGCTGCTACCCGACCACCATG180.10611956137247967No Hit
TCCCGGCTGACCGCCTGATCGCCAATCCGGGTTGCTATCCGACCACCATG180.10611956137247967No Hit
TTCCGGCTGACCGTCTGATCGCCAATCCGGGCTGCTACCCGACCACCATG180.10611956137247967No Hit
TCCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.10022403018511968No Hit
TCCCGGCCGACCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG170.10022403018511968No Hit
TCCCGGCCGATCGTCTGATTGCCAATCCGGGCTGCTACCCGACCACCATG170.10022403018511968No Hit
TTCCGGCCGACCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACCATG170.10022403018511968No Hit
TTCCGGCTGACCGCCTGATCGCCAATCCGGGCTGCTACCCGACCACCATG170.10022403018511968No Hit
TCCCGGCTGATCGCCTGATCGCCAATCCGGGTTGCTATCCGACCACCATG170.10022403018511968No Hit
TTCCGGCCGATCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACCATG170.10022403018511968No Hit
TCCCGGCCGACCGTCTGATCGCCAATCCGGGCTGCTACCCGACCACCATG170.10022403018511968No Hit
TTCCGGCCGATCGCCTGATCGCCAATCCGGGCTGCTACCCGACCACCATG170.10022403018511968No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)