FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20597
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG2231.0826819439724233No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1970.9564499684420061No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1860.9030441326406757No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1800.8739136767490411No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1760.8544933728212847No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1670.8107976889838325No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1650.8010875370199544No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1600.7768121571102587No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC1590.7719570811283196No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1550.7525367772005632No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT1520.7379715492547458No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1500.7282613972908676No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1490.7234063213089285No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1470.7136961693450503No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1460.7088410933631112No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1460.7088410933631112No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1460.7088410933631112No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1410.6845657134534155No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1410.6845657134534155No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1400.6797106374714764No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1390.6748555614895373No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1390.6748555614895373No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1350.6554352575617808No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC1340.6505801815798417No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1340.6505801815798417No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1340.6505801815798417No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC1320.6408700296159635No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1320.6408700296159635No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC1310.6360149536340244No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT1310.6360149536340244No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1280.621449725688207No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC1270.6165946497062679No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC1250.6068844977423896No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT1240.6020294217604505No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT1230.5971743457785115No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1210.5874641938146332No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1210.5874641938146332No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1200.5826091178326941No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1190.577754041850755No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG1190.577754041850755No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT1180.5728989658688158No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG1180.5728989658688158No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC1160.5631888139049376No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT1140.5534786619410594No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC1120.5437685099771812No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC1120.5437685099771812No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC1060.5146380540855464No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT1050.5097829781036073No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT1050.5097829781036073No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT1040.5049279021216683No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT1030.5000728261397291No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC1000.4855075981939117No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC990.48065252221197263No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT990.48065252221197263No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG970.4709423702480944No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC970.4709423702480944No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT950.4612322182842162No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT940.456377142302277No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT940.456377142302277No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT940.456377142302277No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT930.45152206632033787No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC920.4466669903383988No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC920.4466669903383988No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC920.4466669903383988No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC910.44181191435645967No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC900.43695683837452054No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC900.43695683837452054No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC860.41753653444676403No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC850.41268145846482496No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT850.41268145846482496No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG840.40782638248288583No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG830.4029713065009467No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC830.4029713065009467No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC830.4029713065009467No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG820.39811623051900763No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC820.39811623051900763No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC820.39811623051900763No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC810.3932611545370685No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT810.3932611545370685No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC800.3884060785551294No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT790.38355100257319025No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT780.3786959265912512No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG770.37384085060931205No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT770.37384085060931205No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC760.3689857746273729No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT750.3641306986454338No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC740.35927562266349466No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT740.35927562266349466No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT730.3544205466815556No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT730.3544205466815556No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT710.34471039471767734No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG700.3398553187357382No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT700.3398553187357382No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG690.3350002427537991No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT690.3350002427537991No Hit
TCCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG680.33014516677186No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG680.33014516677186No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC680.33014516677186No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG670.3252900907899208No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT670.3252900907899208No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG660.32043501480798176No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG660.32043501480798176No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT650.3155799388260426No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC650.3155799388260426No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG650.3155799388260426No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT650.3155799388260426No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG640.3107248628441035No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC620.30101471088022524No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG620.30101471088022524No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG610.2961596348982862No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG600.29130455891634705No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG600.29130455891634705No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG580.2815944069524688No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG570.2767393309705297No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT560.2718842549885906No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG540.26217410302471233No Hit
TTCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG540.26217410302471233No Hit
TCCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG520.25246395106083414No Hit
TTCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA500.24275379909695585No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG490.23789872311501675No Hit
TTCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA490.23789872311501675No Hit
TCCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG490.23789872311501675No Hit
TTCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG480.23304364713307765No Hit
TTCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA470.2281885711511385No Hit
TCCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA460.2233334951691994No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG450.21847841918726027No Hit
TTCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA440.21362334320532117No Hit
TTCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA430.20876826722338201No Hit
TTCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG420.20391319124144291No Hit
TCCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG420.20391319124144291No Hit
TCCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG420.20391319124144291No Hit
TTCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA410.19905811525950382No Hit
TTCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG410.19905811525950382No Hit
TTCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA400.1942030392775647No Hit
TCCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA400.1942030392775647No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG390.1893479632956256No Hit
TCCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA390.1893479632956256No Hit
TTCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA390.1893479632956256No Hit
TTCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA380.18449288731368646No Hit
TTCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA380.18449288731368646No Hit
TCCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA380.18449288731368646No Hit
TCCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA380.18449288731368646No Hit
TCCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA370.17963781133174733No Hit
TTCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA360.17478273534980823No Hit
TCCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA350.1699276593678691No Hit
TCCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA350.1699276593678691No Hit
TTCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA350.1699276593678691No Hit
TTCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA320.15536243142205175No Hit
TCCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA310.15050735544011262No Hit
TCCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG310.15050735544011262No Hit
TCCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA310.15050735544011262No Hit
TTCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA310.15050735544011262No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC300.14565227945817352No Hit
TTCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA300.14565227945817352No Hit
TTCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA290.1407972034762344No Hit
TCCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA260.12623197553041707No Hit
TCCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA260.12623197553041707No Hit
TCCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA260.12623197553041707No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC260.12623197553041707No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC260.12623197553041707No Hit
TCCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA240.11652182356653883No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC240.11652182356653883No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC240.11652182356653883No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC220.10681167160266059No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC210.10195659562072146No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC210.10195659562072146No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC210.10195659562072146No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)