FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10004
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC1061.0595761695321873No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC1000.9996001599360257No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC880.8796481407437026No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC860.8596561375449819No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC850.8496601359456217No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT790.7896841263494603No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC790.7896841263494603No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC780.7796881247501No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC760.7596961215513794No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT760.7596961215513794No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC760.7596961215513794No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC760.7596961215513794No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT740.7397041183526589No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC730.7297081167532987No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT730.7297081167532987No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT730.7297081167532987No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC730.7297081167532987No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC720.7197121151539384No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC690.6897241103558577No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC660.659736105557777No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC660.659736105557777No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC660.659736105557777No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC660.659736105557777No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC650.6497401039584166No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC650.6497401039584166No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT640.6397441023590564No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC630.6297481007596961No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC630.6297481007596961No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT620.6197520991603359No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT620.6197520991603359No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT600.5997600959616154No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC590.5897640943622551No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT590.5897640943622551No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT590.5897640943622551No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC590.5897640943622551No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT580.5797680927628949No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT580.5797680927628949No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC580.5797680927628949No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC570.5697720911635346No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT570.5697720911635346No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT550.549780087964814No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT550.549780087964814No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC540.5397840863654538No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT540.5397840863654538No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT520.5197920831667333No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT510.509796081567373No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT510.509796081567373No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT510.509796081567373No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC500.49980007996801284No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT490.4898040783686526No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC480.47980807676929227No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT480.47980807676929227No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT470.469812075169932No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC460.4598160735705718No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT460.4598160735705718No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT450.44982007197121154No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC450.44982007197121154No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT450.44982007197121154No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT440.4398240703718513No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT410.4098360655737705No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC410.4098360655737705No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT350.349860055977609No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT340.3398640543782487No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT330.3298680527788885No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG320.3198720511795282No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG310.30987604958016796No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG300.2998800479808077No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG280.2798880447820872No Hit
TTCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG280.2798880447820872No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG280.2798880447820872No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG270.2698920431827269No Hit
TTCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG270.2698920431827269No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG270.2698920431827269No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG260.25989604158336665No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG250.24990003998400642No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG250.24990003998400642No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG250.24990003998400642No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG240.23990403838464613No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC240.23990403838464613No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG240.23990403838464613No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG240.23990403838464613No Hit
TCCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG230.2299080367852859No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG230.2299080367852859No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.21991203518592564No Hit
TCCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.21991203518592564No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.21991203518592564No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG220.21991203518592564No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG210.2099160335865654No Hit
TTCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG210.2099160335865654No Hit
TCCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG210.2099160335865654No Hit
TTCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG200.19992003198720512No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.18992403038784486No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG180.1799280287884846No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC180.1799280287884846No Hit
TCCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG180.1799280287884846No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC170.16993202718912434No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC170.16993202718912434No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.1599360255897641No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC160.1599360255897641No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC160.1599360255897641No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC160.1599360255897641No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC160.1599360255897641No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC150.14994002399040385No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC140.1399440223910436No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC140.1399440223910436No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC140.1399440223910436No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC130.12994802079168333No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC130.12994802079168333No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC130.12994802079168333No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC120.11995201919232307No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC120.11995201919232307No Hit
TTCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGATAGAAACAGACA120.11995201919232307No Hit
TCCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.11995201919232307No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC120.11995201919232307No Hit
TCCCGGCTGATCGCCTGATTGCCACCCACCTGCACGCCCCACTAAGAAGG110.10995601759296282No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC110.10995601759296282No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)