FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10027
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC520.5185997805924005No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC510.5086267078887005No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC510.5086267078887005No Hit
TTCCGGCTGATCGTCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT510.5086267078887005No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC500.49865363518500044No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC490.48868056248130054No Hit
TTCCGGCTGACCGTCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT470.4687344170739005No Hit
TCCCGGCCGATCGTCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT470.4687344170739005No Hit
TTCCGGCTGACCGCCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT470.4687344170739005No Hit
TTCCGGCCGATCGCCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT460.4587613443702005No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC460.4587613443702005No Hit
TCCCGGCTGACCGCCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT450.4487882716665005No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC450.4487882716665005No Hit
TCCCGGCTGATCGTCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT450.4487882716665005No Hit
TTCCGGCTGACCGCCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT440.4388151989628004No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT440.4388151989628004No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC430.4288421262591004No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT430.4288421262591004No Hit
TTCCGGCCGATCGTCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT430.4288421262591004No Hit
TTCCGGCTGATCGCCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT430.4288421262591004No Hit
TCCCGGCTGATCGCCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT420.4188690535554004No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT420.4188690535554004No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC420.4188690535554004No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT420.4188690535554004No Hit
TTCCGGCCGATCGTCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT410.40889598085170037No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC400.39892290814800035No Hit
TTCCGGCTGATCGTCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT400.39892290814800035No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT400.39892290814800035No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT400.39892290814800035No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT400.39892290814800035No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC390.3889498354443004No Hit
TCCCGGCTGATCGCCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT390.3889498354443004No Hit
TCCCGGCCGACCGCCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT390.3889498354443004No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT390.3889498354443004No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT380.3789767627406004No Hit
TTCCGGCCGATCGCCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT360.35903061733320035No Hit
TTCCGGCTGACCGTCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT360.35903061733320035No Hit
TCCCGGCTGACCGTCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT350.34905754462950034No Hit
TTCCGGCCGACCGTCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT350.34905754462950034No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC350.34905754462950034No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT350.34905754462950034No Hit
TTCCGGCTGATCGCCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT340.3390844719258003No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT340.3390844719258003No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT340.3390844719258003No Hit
TTCCGGCCGACCGCCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT340.3390844719258003No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC340.3390844719258003No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC330.3291113992221003No Hit
TTCCGGCCGACCGTCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT330.3291113992221003No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT330.3291113992221003No Hit
TCCCGGCTGACCGTCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT330.3291113992221003No Hit
TCCCGGCCGATCGCCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT330.3291113992221003No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.3291113992221003No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT330.3291113992221003No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC330.3291113992221003No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC320.3191383265184003No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC320.3191383265184003No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC320.3191383265184003No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT320.3191383265184003No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.3191383265184003No Hit
TCCCGGCTGATCGTCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT310.3091652538147003No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT310.3091652538147003No Hit
TCCCGGCCGACCGCCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT310.3091652538147003No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC300.2991921811110003No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT300.2991921811110003No Hit
TCCCGGCCGATCGCCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT290.2892191084073003No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC290.2892191084073003No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.2892191084073003No Hit
TTCCGGCCGACCGCCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT290.2892191084073003No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC290.2892191084073003No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT290.2892191084073003No Hit
TCCCGGCTGACCGCCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT290.2892191084073003No Hit
TCCCGGCCGATCGTCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT290.2892191084073003No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC280.2792460357036003No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT280.2792460357036003No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT280.2792460357036003No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC280.2792460357036003No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.2692729629999003No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.2692729629999003No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT270.2692729629999003No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT260.25929989029620026No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC260.25929989029620026No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC260.25929989029620026No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC260.25929989029620026No Hit
TCCCGGCCGACCGTCTGATTGCCACCTGTGGAGGTACGATTTCAACCAAT250.24932681759250022No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT240.23935374488880026No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC230.22938067218510025No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT230.22938067218510025No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.2194075994814002No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT220.2194075994814002No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT220.2194075994814002No Hit
TCCCGGCCGACCGTCTGATCGCCACCTGTGGAGGTACGATTTCAACCAAT220.2194075994814002No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.2094345267777002No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC200.19946145407400018No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC200.19946145407400018No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC190.1894883813703002No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG190.1894883813703002No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG180.17951530866660018No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG180.17951530866660018No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.16954223596290016No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG170.16954223596290016No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG170.16954223596290016No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.15956916325920015No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG160.15956916325920015No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG150.14959609055550016No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG150.14959609055550016No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG150.14959609055550016No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG150.14959609055550016No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.13962301785180015No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG140.13962301785180015No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG140.13962301785180015No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG140.13962301785180015No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG140.13962301785180015No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC140.13962301785180015No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG130.12964994514810013No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG130.12964994514810013No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.12964994514810013No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.11967687244440013No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG120.11967687244440013No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG120.11967687244440013No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG120.11967687244440013No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC120.11967687244440013No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG120.11967687244440013No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG120.11967687244440013No Hit
TCCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.11967687244440013No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG120.11967687244440013No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG120.11967687244440013No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG120.11967687244440013No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.11967687244440013No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC120.11967687244440013No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG120.11967687244440013No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG110.1097037997407001No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG110.1097037997407001No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG110.1097037997407001No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCCGTG110.1097037997407001No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC110.1097037997407001No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC110.1097037997407001No Hit
TTCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG110.1097037997407001No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG110.1097037997407001No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCCGTG110.1097037997407001No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC110.1097037997407001No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG110.1097037997407001No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC110.1097037997407001No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG110.1097037997407001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)