FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13348
Sequences flagged as poor quality0
Sequence length301
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2892.1651183697932277No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2862.142643092598142No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2682.0077914294276296No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2551.9103985615822594No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA2511.8804315253221455No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2481.85795624812706No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA2441.8279892118669463No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA2431.820497452801918No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA2371.775546898411747No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2301.7231045849565478No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2271.7006293077614625No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA2241.678154030566377No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA2181.6332034761762062No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA2111.5807611627210068No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2111.5807611627210068No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA2071.550794126460893No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA2051.535810608330836No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA2021.5133353311357507No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1991.4908600539406653No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1941.453401258615523No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1921.438417740485466No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1921.438417740485466No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1901.423434222355409No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1871.4009589451603237No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1831.3709919089002098No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1831.3709919089002098No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1761.3185495954450104No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1751.3110578363799819No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1751.3110578363799819No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1701.2735990410548397No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA1551.1612226550794127No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA1541.1537308960143842No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC500.3745879532514234No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC500.3745879532514234No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC430.3221456397962241No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC430.3221456397962241No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC420.31465388073119566No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC420.31465388073119566No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC410.30716212166616724No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT410.30716212166616724No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC410.30716212166616724No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC400.2996703626011388No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC380.28468684447108183No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC370.2771950854060533No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC360.26970332634102484No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC360.26970332634102484No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT360.26970332634102484No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT350.2622115672759964No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC350.2622115672759964No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC340.25471980821096796No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT340.25471980821096796No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT340.25471980821096796No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC330.2472280491459395No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.2472280491459395No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC330.2472280491459395No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC320.23973629008091102No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC320.23973629008091102No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC320.23973629008091102No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.23973629008091102No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC320.23973629008091102No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT310.2322445310158825No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT310.2322445310158825No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC300.22475277195085405No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC280.2097692538207971No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC280.2097692538207971No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT280.2097692538207971No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT280.2097692538207971No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.20227749475576867No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.20227749475576867No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC270.20227749475576867No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC260.1947857356907402No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT260.1947857356907402No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC260.1947857356907402No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT260.1947857356907402No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC260.1947857356907402No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT260.1947857356907402No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.1872939766257117No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC250.1872939766257117No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.1872939766257117No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT250.1872939766257117No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.1872939766257117No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.1872939766257117No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG240.17980221756068324No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT240.17980221756068324No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT240.17980221756068324No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC220.1648186994306263No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT220.1648186994306263No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT220.1648186994306263No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.15732694036559783No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.15732694036559783No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.15732694036559783No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.15732694036559783No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC200.1498351813005694No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG200.1498351813005694No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.14234342223554092No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG180.13485166317051242No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.12735990410548398No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.12735990410548398No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.12735990410548398No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.11986814504045551No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.11237638597542703No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG150.11237638597542703No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.10488462691039856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)