FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9571
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1491.5567861247518546No Hit
TTCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1451.5149932086511335No Hit
TTCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1411.4732002925504128No Hit
TCCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1321.3791662313237907No Hit
TTCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1291.34782154424825No Hit
TCCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1241.2955803991223487No Hit
TTCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1231.2851321700971685No Hit
TTCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1191.2433392539964476No Hit
TCCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1121.170201650820186No Hit
TTCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1091.1388569637446453No Hit
TTCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1081.128408734719465No Hit
TTCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1071.1179605056942847No Hit
TCCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1051.0970640476439244No Hit
TCCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1041.086615818618744No Hit
TCCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1031.0761675895935638No Hit
TCCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1011.0552711315432033No Hit
TTCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA991.034374673492843No Hit
TTCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA981.0239264444676626No Hit
TCCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA971.0134782154424826No Hit
TCCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA961.0030299864173022No Hit
TTCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA950.992581757392122No Hit
TCCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA940.9821335283669418No Hit
TTCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA910.9507888412914011No Hit
TCCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA910.9507888412914011No Hit
TCCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA890.9298923832410407No Hit
TTCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA880.9194441542158603No Hit
TCCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA860.8985476961655No Hit
TCCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA860.8985476961655No Hit
TTCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA810.8463065510395988No Hit
TCCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA790.8254100929892384No Hit
TCCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA780.8149618639640581No Hit
TTCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA780.8149618639640581No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA730.762720718838157No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA610.6373419705359942No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA610.6373419705359942No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA570.5955490544352732No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA540.5642043673597325No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA540.5642043673597325No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA540.5642043673597325No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA490.5119632222338313No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA490.5119632222338313No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA480.5015149932086511No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA480.5015149932086511No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA470.4910667641834709No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA470.4910667641834709No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA460.4806185351582907No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA460.4806185351582907No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA450.4701703061331104No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA440.45972207710793017No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA440.45972207710793017No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA440.45972207710793017No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA430.44927384808275No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA430.44927384808275No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA430.44927384808275No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA390.40748093198202906No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA390.40748093198202906No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA390.40748093198202906No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA380.39703270295684884No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA380.39703270295684884No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA370.38658447393166856No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA370.38658447393166856No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA360.3761362449064884No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA300.31344687075540695No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT240.25075749660432556No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.22986103855396509No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC220.22986103855396509No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT220.22986103855396509No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT220.22986103855396509No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA210.2194128095287849No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.2194128095287849No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.19851635147842442No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.1880681224532442No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.1880681224532442No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC170.17761989342806395No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC170.17761989342806395No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.17761989342806395No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT170.17761989342806395No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.17761989342806395No Hit
TTCCGGCTGATCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG160.16717166440288372No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC160.16717166440288372No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT160.16717166440288372No Hit
TTCCGGCTGATCGCCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG160.16717166440288372No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT160.16717166440288372No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.15672343537770347No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT150.15672343537770347No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC150.15672343537770347No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC140.14627520635252325No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT140.14627520635252325No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC140.14627520635252325No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT140.14627520635252325No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT140.14627520635252325No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC140.14627520635252325No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT130.135826977327343No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT130.135826977327343No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC130.135826977327343No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT130.135826977327343No Hit
TTCCGGCCGATCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG130.135826977327343No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC130.135826977327343No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC130.135826977327343No Hit
TTCCGGCCGACCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACGATG130.135826977327343No Hit
TCCCGGCTGATCGTCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG120.12537874830216278No Hit
TTCCGGCTGATCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACGATG120.12537874830216278No Hit
TCCCGGCTGATCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACGATG120.12537874830216278No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC120.12537874830216278No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT120.12537874830216278No Hit
TTCCGGCCGATCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACGATG120.12537874830216278No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC120.12537874830216278No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC120.12537874830216278No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.12537874830216278No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT120.12537874830216278No Hit
TCCCGGCTGATCGCCTGATTGCCAATCCGGGTTGCTACCCGACCACGATG120.12537874830216278No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT110.11493051927698254No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC110.11493051927698254No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC110.11493051927698254No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG110.11493051927698254No Hit
TCCCGGCTGATCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG110.11493051927698254No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC110.11493051927698254No Hit
TCCCGGCCGATCGCCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG110.11493051927698254No Hit
TTCCGGCTGACCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACGATG110.11493051927698254No Hit
TTCCGGCCGATCGCCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG110.11493051927698254No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG110.11493051927698254No Hit
TTCCGGCTGATCGCCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG110.11493051927698254No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC110.11493051927698254No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC110.11493051927698254No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC100.10448229025180232No Hit
TCCCGGCTGATCGTCTGATCGCCAATCCGGGTTGCTACCCGACCACGATG100.10448229025180232No Hit
TCCCGGCCGACCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG100.10448229025180232No Hit
TTCCGGCTGACCGTCTGATCGCCAATCCGGGTTGCTACCCGACCACGATG100.10448229025180232No Hit
TCCCGGCCGATCGCCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG100.10448229025180232No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG100.10448229025180232No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC100.10448229025180232No Hit
TCCCGGCTGACCGCCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG100.10448229025180232No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC100.10448229025180232No Hit
TCCCGGCTGACCGCCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG100.10448229025180232No Hit
TCCCGGCCGACCGCCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG100.10448229025180232No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT100.10448229025180232No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC100.10448229025180232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)