FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12003
Sequences flagged as poor quality0
Sequence length301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG1040.8664500541531284No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG950.7914687994667999No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG800.6665000416562525No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG790.6581687911355495No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA770.6415062900941432No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG750.6248437890527367No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG740.6165125385320337No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG720.5998500374906273No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG700.5831875364492211No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA670.5581937848871116No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG670.5581937848871116No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG640.533200033325002No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG630.5248687828042989No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG630.5248687828042989No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG630.5248687828042989No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG610.5082062817628926No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG600.49987503124218946No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA600.49987503124218946No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG600.49987503124218946No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG590.49154378072148625No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA580.48321253020078314No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA580.48321253020078314No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA570.47488127968008No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG570.47488127968008No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG570.47488127968008No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG560.4665500291593769No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG540.4498875281179705No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG540.4498875281179705No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA530.4415562775972674No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA530.4415562775972674No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA520.4332250270765642No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA520.4332250270765642No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA520.4332250270765642No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA500.41656252603515787No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG500.41656252603515787No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA500.41656252603515787No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG500.41656252603515787No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA500.41656252603515787No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG490.4082312755144547No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG490.4082312755144547No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG490.4082312755144547No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG490.4082312755144547No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA480.3999000249937516No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA480.3999000249937516No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG470.3915687744730484No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA460.3832375239523453No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA450.3749062734316421No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA440.3665750229109389No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA440.3665750229109389No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA440.3665750229109389No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA410.3415812713488295No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA410.3415812713488295No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA400.33325002082812627No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA400.33325002082812627No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAACGGGGTGATGAATGCCGTG390.3249187703074231No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA390.3249187703074231No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA380.31658751978672No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA380.31658751978672No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG380.31658751978672No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGGGTGATGAATGCCGTG380.31658751978672No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA370.30825626926601685No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC310.2582687661417979No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA310.2582687661417979No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA300.24993751562109473No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA300.24993751562109473No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC290.24160626510039157No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC290.24160626510039157No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC290.24160626510039157No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC280.23327501457968844No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC270.22494376405898525No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC260.2166125135382821No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT250.20828126301757893No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC250.20828126301757893No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.20828126301757893No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC240.1999500124968758No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC240.1999500124968758No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC240.1999500124968758No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC230.19161876197617264No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC230.19161876197617264No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC230.19161876197617264No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT230.19161876197617264No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT230.19161876197617264No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT220.18328751145546945No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT220.18328751145546945No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC220.18328751145546945No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC220.18328751145546945No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.17495626093476632No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.17495626093476632No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT210.17495626093476632No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTGATGAATGCGGTG210.17495626093476632No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.17495626093476632No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.17495626093476632No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC200.16662501041406313No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAATGGCGTGATGAATGCGGTG200.16662501041406313No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAATGGCGTGATGAATGCGGTG200.16662501041406313No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT200.16662501041406313No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT200.16662501041406313No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT200.16662501041406313No Hit
TTCCGGCCGATCGTCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA190.15829375989336No Hit
TTCCGGCTGATCGTCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA180.14996250937265682No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAATGGCGTGATGAATGCGGTG180.14996250937265682No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT180.14996250937265682No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG180.14996250937265682No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTCATGAACGCTGTG180.14996250937265682No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAATGGCGTGATGAATGCGGTG180.14996250937265682No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC170.14163125885195368No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAATGGCGTGATGAATGCGGTG170.14163125885195368No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC170.14163125885195368No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAATGGCGTGATGAATGCGGTG170.14163125885195368No Hit
TTCCGGCTGATCGCCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA160.1333000083312505No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.1333000083312505No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT160.1333000083312505No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC160.1333000083312505No Hit
TTCCGGCTGATCGTCTGATTGCCAATCCAGGTTGCTACCCGACCACGATG160.1333000083312505No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.1333000083312505No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT160.1333000083312505No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG150.12496875781054737No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC150.12496875781054737No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAATGGCGTGATGAATGCGGTG150.12496875781054737No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAATGGCGTGATGAATGCGGTG150.12496875781054737No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT150.12496875781054737No Hit
TTCCGGCTGACCGCCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA150.12496875781054737No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC150.12496875781054737No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.12496875781054737No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT150.12496875781054737No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.12496875781054737No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTGATGAATGCGGTG150.12496875781054737No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC150.12496875781054737No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.11663750728984422No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT140.11663750728984422No Hit
TCCCGGCTGATCGTCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA140.11663750728984422No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTGATGAATGCGGTG140.11663750728984422No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC140.11663750728984422No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT140.11663750728984422No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT140.11663750728984422No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC140.11663750728984422No Hit
TTCCGGCTGATCGCCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA140.11663750728984422No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAATGGCGTGATGAATGCGGTG140.11663750728984422No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.11663750728984422No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAATGGCGTGATGAATGCGGTG140.11663750728984422No Hit
TCCCGGCCGATCGTCTGATTGCCAATTTTACATGGTGTTTCCGCCCTCGA130.10830625676914105No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAATGGCGTGATGAATGCGGTG130.10830625676914105No Hit
TCCCGGCTGACCGTCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA130.10830625676914105No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC130.10830625676914105No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAATGGCGTGATGAATGCGGTG130.10830625676914105No Hit
TCCCGGCTGACCGTCTGATCGCCAATCCAGGTTGCTACCCGACCACGATG130.10830625676914105No Hit
TTCCGGCTGATCGTCTGATCGCCAATTTTACATGGTGTTTCCGCCCTCGA130.10830625676914105No Hit
TCCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.10830625676914105No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)