FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10654
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1811.6988924347662848No Hit
TTCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1591.4923972217007697No Hit
TTCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1261.1826544021024967No Hit
TCCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1251.1732682560540642No Hit
TCCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1191.1169513797634691No Hit
TCCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1151.079406795569739No Hit
TTCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1151.079406795569739No Hit
TTCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1111.041862211376009No Hit
TTCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1091.023089919279144No Hit
TCCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1081.0137037732307115No Hit
TTCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1071.004317627182279No Hit
TTCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1071.004317627182279No Hit
TTCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1060.9949314811338464No Hit
TTCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1040.9761591890369814No Hit
TTCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1030.9667730429885489No Hit
TCCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1020.9573868969401165No Hit
TCCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1020.9573868969401165No Hit
TCCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA960.9010700206495214No Hit
TCCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA960.9010700206495214No Hit
TTCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA930.8729115825042238No Hit
TTCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA910.8541392904073587No Hit
TTCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA900.8447531443589262No Hit
TCCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA900.8447531443589262No Hit
TCCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA900.8447531443589262No Hit
TCCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA830.7790501220198986No Hit
TTCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA820.7696639759714661No Hit
TCCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA820.7696639759714661No Hit
TTCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA810.7602778299230336No Hit
TCCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA790.7415055378261686No Hit
TCCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA780.732119391777736No Hit
TCCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA750.7039609536324386No Hit
TCCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA730.6851886615355735No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT370.347287403792003No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC340.31912896564670545No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC330.30974281959827293No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT300.28158438145297543No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.27219823540454285No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC290.27219823540454285No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.27219823540454285No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT280.2628120893561104No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT280.2628120893561104No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT280.2628120893561104No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC270.25342594330767787No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT270.25342594330767787No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT270.25342594330767787No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT270.25342594330767787No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC260.24403979725924535No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT260.24403979725924535No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC250.23465365121081283No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC250.23465365121081283No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT250.23465365121081283No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT250.23465365121081283No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT240.22526750516238034No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT240.22526750516238034No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT240.22526750516238034No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT230.2158813591139478No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT230.2158813591139478No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC230.2158813591139478No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG220.2064952130655153No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC220.2064952130655153No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT220.2064952130655153No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT220.2064952130655153No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC210.19710906701708278No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.19710906701708278No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT210.19710906701708278No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG210.19710906701708278No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG200.1877229209686503No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC200.1877229209686503No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC200.1877229209686503No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.17833677492021777No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.17833677492021777No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.17833677492021777No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG180.16895062887178525No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC180.16895062887178525No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC180.16895062887178525No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC180.16895062887178525No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.15956448282335273No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.15956448282335273No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.15956448282335273No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.15956448282335273No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.1501783367749202No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC160.1501783367749202No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.1501783367749202No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.1501783367749202No Hit
TTCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.1501783367749202No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.1501783367749202No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.1501783367749202No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC150.14079219072648771No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC150.14079219072648771No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.14079219072648771No Hit
TCCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.14079219072648771No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC150.14079219072648771No Hit
TTCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.14079219072648771No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.1314060446780552No Hit
TCCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.1314060446780552No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAATGGTGTGATGAACGCTGTG130.12201989862962268No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT130.12201989862962268No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC130.12201989862962268No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC130.12201989862962268No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.12201989862962268No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC130.12201989862962268No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT130.12201989862962268No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC130.12201989862962268No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG130.12201989862962268No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT130.12201989862962268No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.12201989862962268No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT120.11263375258119017No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC120.11263375258119017No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC120.11263375258119017No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.11263375258119017No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC120.11263375258119017No Hit
TTCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG110.10324760653275765No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG110.10324760653275765No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAATGGTGTGATGAACGCTGTG110.10324760653275765No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG110.10324760653275765No Hit
TTCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG110.10324760653275765No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG110.10324760653275765No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG110.10324760653275765No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG110.10324760653275765No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT110.10324760653275765No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG110.10324760653275765No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCAGTG110.10324760653275765No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)