FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10393
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA590.5676897912056192No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA550.5292023477340517No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA540.51958048686616No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA530.5099586259982681No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA520.5003367651303763No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA510.4907149042624843No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA480.4618493216588088No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA470.45222746079091697No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA470.45222746079091697No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA460.4426055999230251No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA440.42336187818724136No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA430.41374001731934956No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA420.4041181564514577No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA400.384874434715674No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA390.37525257384778216No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA390.37525257384778216No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA390.37525257384778216No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA380.3656307129798903No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA360.3463869912441066No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA350.33676513037621475No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA350.33676513037621475No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA340.3271432695083229No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA340.3271432695083229No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA340.3271432695083229No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA340.3271432695083229No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA290.27903396516886364No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC290.27903396516886364No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.27903396516886364No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA290.27903396516886364No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC280.2694121043009718No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA270.25979024343308No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA270.25979024343308No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC270.25979024343308No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA260.25016838256518814No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT260.25016838256518814No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG250.24054652169729623No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC250.24054652169729623No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA250.24054652169729623No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA250.24054652169729623No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC250.24054652169729623No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG250.24054652169729623No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG240.2309246608294044No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC240.2309246608294044No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC240.2309246608294044No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC240.2309246608294044No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT240.2309246608294044No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG230.22130279996151256No Hit
TCCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.21168093909362068No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG220.21168093909362068No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.21168093909362068No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG220.21168093909362068No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC220.21168093909362068No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT220.21168093909362068No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.21168093909362068No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC210.20205907822572886No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG210.20205907822572886No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG210.20205907822572886No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC210.20205907822572886No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC200.192437217357837No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC200.192437217357837No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT200.192437217357837No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT200.192437217357837No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC200.192437217357837No Hit
TCCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG200.192437217357837No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC200.192437217357837No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT200.192437217357837No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG200.192437217357837No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.18281535648994515No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC190.18281535648994515No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC190.18281535648994515No Hit
TTCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG190.18281535648994515No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.18281535648994515No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT190.18281535648994515No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.18281535648994515No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT190.18281535648994515No Hit
TTCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG180.1731934956220533No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG180.1731934956220533No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG180.1731934956220533No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC180.1731934956220533No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.1731934956220533No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.1731934956220533No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT180.1731934956220533No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.16357163475416145No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT170.16357163475416145No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.16357163475416145No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.16357163475416145No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.16357163475416145No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC170.16357163475416145No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT170.16357163475416145No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.16357163475416145No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC160.1539497738862696No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.1539497738862696No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.1539497738862696No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT160.1539497738862696No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.1539497738862696No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.1539497738862696No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT160.1539497738862696No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT160.1539497738862696No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.1539497738862696No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.14432791301837777No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC150.14432791301837777No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG150.14432791301837777No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT150.14432791301837777No Hit
TCCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.14432791301837777No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC140.1347060521504859No Hit
TCCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.1347060521504859No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT140.1347060521504859No Hit
TCCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.1347060521504859No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC140.1347060521504859No Hit
TTCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.1347060521504859No Hit
TCCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.1347060521504859No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.12508419128259407No Hit
TTCCGGCTGATCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG130.12508419128259407No Hit
TTCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.12508419128259407No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC130.12508419128259407No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT130.12508419128259407No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT130.12508419128259407No Hit
TTCCGGCTGACCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG130.12508419128259407No Hit
TCCCGGCCGACCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG120.1154623304147022No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.1154623304147022No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC120.1154623304147022No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT120.1154623304147022No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT120.1154623304147022No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT120.1154623304147022No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT120.1154623304147022No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC120.1154623304147022No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC110.10584046954681034No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC110.10584046954681034No Hit
TTCCGGCTGATCGTCTGATTGCCAATCCGGGTTGCTATCCGACCACGATG110.10584046954681034No Hit
TCCCGGCTGATCGTCTGATCGCCAATCCGGGTTGCTATCCGACCACGATG110.10584046954681034No Hit
TCCCGGCTGATCGCCTGATTGCCAATCCGGGTTGCTATCCGACCACCATG110.10584046954681034No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)