FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10124
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC780.7704464638482813No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC770.7605689450809957No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC760.75069142631371No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT740.7309363887791387No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC740.7309363887791387No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC740.7309363887791387No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT730.7210588700118531No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC690.6815487949427104No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT670.6617937574081391No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT670.6617937574081391No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC660.6519162386408534No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT660.6519162386408534No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT660.6519162386408534No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC650.6420387198735678No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT630.6222836823389964No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC620.6124061635717107No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC610.6025286448044251No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT610.6025286448044251No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT600.5926511260371394No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT590.5827736072698538No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC590.5827736072698538No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT590.5827736072698538No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC580.5728960885025681No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT570.5630185697352825No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC560.5531410509679968No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC550.5432635322007112No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC540.5333860134334255No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC540.5333860134334255No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC530.5235084946661399No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC520.5136309758988542No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC510.5037534571315685No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC510.5037534571315685No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT510.5037534571315685No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC510.5037534571315685No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT500.49387593836428295No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC500.49387593836428295No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC500.49387593836428295No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT490.48399841959699724No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC490.48399841959699724No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT490.48399841959699724No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC490.48399841959699724No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT480.4741209008297116No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT480.4741209008297116No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC470.46424338206242594No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT460.4543658632951403No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT450.4444883445278546No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT450.4444883445278546No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT450.4444883445278546No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC450.4444883445278546No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT450.4444883445278546No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC440.434610825760569No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT440.434610825760569No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT440.434610825760569No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC420.41485578822599767No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC420.41485578822599767No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT420.41485578822599767No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT410.40497826945871196No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC390.38522323192414065No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT390.38522323192414065No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT380.375345713156855No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC370.36546819438956935No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT350.34571315685499804No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.3259581193204267No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT330.3259581193204267No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG310.30620308178585537No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG300.2963255630185697No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG290.28644804425128406No Hit
TCCCGGCTGACCGCCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG270.26669300671671275No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG270.26669300671671275No Hit
TTCCGGCTGACCGCCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG260.2568154879494271No Hit
TCCCGGCTGATCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG240.2370604504148558No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG240.2370604504148558No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG230.22718293164757014No Hit
TTCCGGCTGATCGTCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG230.22718293164757014No Hit
TCCCGGCTGATCGCCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG220.2173054128802845No Hit
TTCCGGCCGATCGTCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG220.2173054128802845No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG220.2173054128802845No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG210.20742789411299883No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG210.20742789411299883No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG210.20742789411299883No Hit
TTCCGGCTGATCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG200.19755037534571318No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG200.19755037534571318No Hit
TCCCGGCCGACCGTCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG200.19755037534571318No Hit
TCCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.1876728565784275No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG190.1876728565784275No Hit
TTCCGGCCGATCGCCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG190.1876728565784275No Hit
TTCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG190.1876728565784275No Hit
TTCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG190.1876728565784275No Hit
TTCCGGCTGACCGCCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG180.17779533781114185No Hit
TCCCGGCTGACCGCCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG180.17779533781114185No Hit
TTCCGGCTGACCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG180.17779533781114185No Hit
TTCCGGCTGATCGCCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG180.17779533781114185No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG170.16791781904385616No Hit
TCCCGGCTGACCGTCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG170.16791781904385616No Hit
TCCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.16791781904385616No Hit
TCCCGGCTGACCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG170.16791781904385616No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.16791781904385616No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.1580403002765705No Hit
TCCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.1580403002765705No Hit
TTCCGGCCGATCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG160.1580403002765705No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.1580403002765705No Hit
TCCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.1580403002765705No Hit
TCCCGGCCGACCGCCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG160.1580403002765705No Hit
TTCCGGCTGATCGCCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG160.1580403002765705No Hit
TTCCGGCCGACCGCCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG150.14816278150928486No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.14816278150928486No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.14816278150928486No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.14816278150928486No Hit
TCCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.14816278150928486No Hit
TCCCGGCCGATCGCCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG150.14816278150928486No Hit
TTCCGGCTGACCGTCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG150.14816278150928486No Hit
TTCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.1382852627419992No Hit
TTCCGGCCGACCGTCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG140.1382852627419992No Hit
TCCCGGCTGATCGTCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG140.1382852627419992No Hit
TCCCGGCCGATCGCCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG140.1382852627419992No Hit
TTCCGGCCGACCGCCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG140.1382852627419992No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG130.12840774397471355No Hit
TTCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG130.12840774397471355No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG120.1185302252074279No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG120.1185302252074279No Hit
TTCCGGCCGACCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG120.1185302252074279No Hit
TCCCGGCCGACCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG110.10865270644014224No Hit
TCCCGGCCGATCGTCTGATTGCCAATCCGGGTTGCTACCCGACCACCATG110.10865270644014224No Hit
TCCCGGCCGATCGTCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG110.10865270644014224No Hit
TCCCGGCTGATCGCCTGATCGCCAATCCGGGTTGCTACCCGACCACCATG110.10865270644014224No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)