FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11457
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT470.41022955398446365No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC420.3665881120712228No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT400.3491315353059265No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC390.34040324692327834No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC380.33167495854063017No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG370.322946670157982No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC370.322946670157982No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC370.322946670157982No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC360.3142183817753339No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC350.3054900933926857No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC350.3054900933926857No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC340.2967618050100375No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT340.2967618050100375No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT340.2967618050100375No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT330.2880335166273894No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT330.2880335166273894No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT330.2880335166273894No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT320.2793052282447412No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC320.2793052282447412No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG310.270576939862093No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG310.270576939862093No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC310.270576939862093No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC310.270576939862093No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC300.2618486514794449No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC300.2618486514794449No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT300.2618486514794449No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT300.2618486514794449No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC280.24439207471414853No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC280.24439207471414853No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.2356637863315004No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.2356637863315004No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.2356637863315004No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT260.22693549794885223No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC260.22693549794885223No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG260.22693549794885223No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC250.21820720956620404No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG250.21820720956620404No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG250.21820720956620404No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC250.21820720956620404No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC250.21820720956620404No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG240.20947892118355593No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT240.20947892118355593No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG240.20947892118355593No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT240.20947892118355593No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC230.20075063280090774No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT230.20075063280090774No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC230.20075063280090774No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT230.20075063280090774No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT230.20075063280090774No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT230.20075063280090774No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG220.19202234441825958No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.19202234441825958No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG220.19202234441825958No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT220.19202234441825958No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC220.19202234441825958No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.19202234441825958No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT220.19202234441825958No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC210.1832940560356114No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG210.1832940560356114No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG210.1832940560356114No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG210.1832940560356114No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.1832940560356114No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG210.1832940560356114No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG200.17456576765296325No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG200.17456576765296325No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG200.17456576765296325No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG200.17456576765296325No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC200.17456576765296325No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG200.17456576765296325No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG200.17456576765296325No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT200.17456576765296325No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC200.17456576765296325No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC190.16583747927031509No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.16583747927031509No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC190.16583747927031509No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG190.16583747927031509No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.16583747927031509No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT190.16583747927031509No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.15710919088766695No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC180.15710919088766695No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.15710919088766695No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG180.15710919088766695No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT180.15710919088766695No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT170.14838090250501876No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.14838090250501876No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG160.1396526141223706No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.1396526141223706No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.1396526141223706No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG160.1396526141223706No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG160.1396526141223706No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG150.13092432573972246No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC150.13092432573972246No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG150.13092432573972246No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG150.13092432573972246No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG150.13092432573972246No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.13092432573972246No Hit
TTCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG150.13092432573972246No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG150.13092432573972246No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG140.12219603735707427No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.12219603735707427No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.12219603735707427No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC140.12219603735707427No Hit
TTCCGGCTGATCGTCTGATTGCCATGACGCAACACCATCGCCAGAAAGTG140.12219603735707427No Hit
TCCCGGCTGACCGTCTGATTGCCATGACGCAACACCATCGCCAGAAAGTG140.12219603735707427No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG140.12219603735707427No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG140.12219603735707427No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG140.12219603735707427No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG140.12219603735707427No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT140.12219603735707427No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG140.12219603735707427No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG140.12219603735707427No Hit
TTCCGGCTGACCGTCTGATCGCCATGACGCAACACCATCGCCAGAAAGTG140.12219603735707427No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.12219603735707427No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.12219603735707427No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAATGGCGTCATGAACGCCGTG130.11346774897442612No Hit
TTCCGGCCGATCGTCTGATTGCCATGACGCAACACCATCGCCAGAAAGTG130.11346774897442612No Hit
TTCCGGCTGATCGCCTGATTGCCAACCCGGGTTGCTACCCGACCACCATG130.11346774897442612No Hit
TCCCGGCTGACCGTCTGATTGCCAACCCGGGTTGCTACCCGACCACCATG130.11346774897442612No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG120.10473946059177797No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.10473946059177797No Hit
TCCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG120.10473946059177797No Hit
TCCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG120.10473946059177797No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG120.10473946059177797No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAATGGCGTCATGAACGCCGTG120.10473946059177797No Hit
TCCCGGCTGATCGTCTGATTGCCATGACGCAACACCATCGCCAGAAAGTG120.10473946059177797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)