FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13735
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1881.3687659264652348No Hit
TTCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1761.2813978886057518No Hit
TCCCGGCTGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1651.2013105205678922No Hit
TTCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1591.1576265016381508No Hit
TCCCGGCTGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1571.1430651619949035No Hit
TTCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1441.0484164543137968No Hit
TCCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1441.0484164543137968No Hit
TCCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1431.0411357844921734No Hit
TCCCGGCTGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1421.0338551146705497No Hit
TTCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1401.0192937750273026No Hit
TCCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1391.0120131052056789No Hit
TTCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1391.0120131052056789No Hit
TTCCGGCCGATCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1370.9974517655624318No Hit
TTCCGGCTGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1300.9464870768110666No Hit
TTCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1280.9319257371678195No Hit
TTCCGGCTGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1280.9319257371678195No Hit
TCCCGGCTGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1260.9173643975245723No Hit
TCCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1230.8955223880597015No Hit
TTCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1230.8955223880597015No Hit
TTCCGGCCGACCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1230.8955223880597015No Hit
TTCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1200.8736803785948308No Hit
TTCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1190.8663997087732072No Hit
TCCCGGCTGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1150.8372770294867127No Hit
TCCCGGCTGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1150.8372770294867127No Hit
TCCCGGCCGATCGCCTGATTGCCAACTGAGATAACAGACAGAAACAGACA1120.815435020021842No Hit
TTCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1110.8081543502002184No Hit
TCCCGGCCGACCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1040.7571896614488532No Hit
TCCCGGCCGACCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA1030.7499089916272297No Hit
TTCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA990.7207863123407354No Hit
TCCCGGCCGACCGTCTGATTGCCAACTGAGATAACAGACAGAAACAGACA990.7207863123407354No Hit
TCCCGGCCGATCGCCTGATCGCCAACTGAGATAACAGACAGAAACAGACA960.6989443028758646No Hit
TCCCGGCCGATCGTCTGATCGCCAACTGAGATAACAGACAGAAACAGACA860.6261376046596286No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC550.4004368401892974No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC510.37131416090280306No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC490.3567528212595559No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC470.3421914816163087No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT470.3421914816163087No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC450.32763014197306156No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC440.32034947215143794No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC440.32034947215143794No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT420.30578813250819076No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT410.2985074626865672No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC400.2912267928649436No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT380.2766654532216964No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC370.26938478340007277No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC360.2621041135784492No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT360.2621041135784492No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC350.25482344375682564No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC350.25482344375682564No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC340.24754277393520202No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT340.24754277393520202No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT340.24754277393520202No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC330.24026210411357846No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC330.24026210411357846No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC330.24026210411357846No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC320.23298143429195486No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC320.23298143429195486No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC320.23298143429195486No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC310.22570076447033124No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT310.22570076447033124No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC310.22570076447033124No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC300.2184200946487077No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT300.2184200946487077No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.2111394248270841No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC290.2111394248270841No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT290.2111394248270841No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.2111394248270841No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.2111394248270841No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT290.2111394248270841No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC280.2038587550054605No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC280.2038587550054605No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT280.2038587550054605No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.19657808518383693No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC270.19657808518383693No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.19657808518383693No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.19657808518383693No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.19657808518383693No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.19657808518383693No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT270.19657808518383693No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT260.1892974153622133No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC250.18201674554058975No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC240.17473607571896616No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT230.16745540589734256No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT230.16745540589734256No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT230.16745540589734256No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.16017473607571897No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC220.16017473607571897No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT220.16017473607571897No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.15289406625409538No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.15289406625409538No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC200.1456133964324718No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.1383327266108482No Hit
TTCCGGCTGACCGCCTGATCGCCACCAAATCAGGATAGCGCCGTATCAAA190.1383327266108482No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.1383327266108482No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT190.1383327266108482No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.1310520567892246No Hit
TTCCGGCTGACCGTCTGATCGCCACCAAATCAGGATAGCGCCGTATCAAA170.12377138696760101No Hit
TCCCGGCTGACCGCCTGATCGCCACCAAATCAGGATAGCGCCGTATCAAA160.11649071714597743No Hit
TTCCGGCCGACCGCCTGATCGCCACCAAATCAGGATAGCGCCGTATCAAA160.11649071714597743No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.11649071714597743No Hit
TCCCGGCTGACCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAAA150.10921004732435385No Hit
TCCCGGCTGATCGCCTGATTGCCACCAAATCAGGATAGCGCCGTATCAAA140.10192937750273025No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG140.10192937750273025No Hit
TCCCGGCTGATCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAAA140.10192937750273025No Hit
TTCCGGCCGATCGTCTGATTGCCACCAAATCAGGATAGCGCCGTATCAAA140.10192937750273025No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)