FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13943
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC1040.7458939969877357No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC970.6956895933443304No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC970.6956895933443304No Hit
TCCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC870.6239690167108944No Hit
TTCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC840.6024528437208635No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT830.5952807860575199No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC830.5952807860575199No Hit
TTCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG810.5809366707308327No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC790.5665925554041454No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC770.5522484400774582No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT760.5450763824141146No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT750.537904324750771No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC740.5307322670874274No Hit
TTCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC730.5235602094240838No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT720.5163881517607402No Hit
TCCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC710.5092160940973965No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC710.5092160940973965No Hit
TCCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC700.5020440364340529No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC690.4948719787707093No Hit
TCCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC690.4948719787707093No Hit
TCCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC690.4948719787707093No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC680.48769992110736565No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC680.48769992110736565No Hit
TTCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC670.48052786344402204No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT640.45901169045399126No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT620.444667575127304No Hit
TTCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC620.444667575127304No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT620.444667575127304No Hit
TTCCGGCCGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC610.43749551746396037No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC600.43032345980061676No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT600.43032345980061676No Hit
TTCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG600.43032345980061676No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC590.4231514021372732No Hit
TTCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT580.4159793444739296No Hit
TTCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG570.408807286810586No Hit
TCCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC570.408807286810586No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT560.40163522914724237No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT550.3944631714838987No Hit
TCCCGGCTGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG550.3944631714838987No Hit
TTCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG550.3944631714838987No Hit
TCCCGGCTGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC550.3944631714838987No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT540.38729111382055514No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT520.37294699849386787No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT510.3657749408305243No Hit
TTCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG510.3657749408305243No Hit
TCCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC500.3586028831671807No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT500.3586028831671807No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT500.3586028831671807No Hit
TTCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC490.35143082550383703No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT490.35143082550383703No Hit
TTCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG490.35143082550383703No Hit
TCCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC480.3442587678404934No Hit
TTCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG480.3442587678404934No Hit
TCCCGGCTGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG470.33708671017714986No Hit
TCCCGGCCGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC470.33708671017714986No Hit
TCCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT460.3299146525138062No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT450.3227425948504626No Hit
TCCCGGCCGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC440.315570537187119No Hit
TTCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG440.315570537187119No Hit
TTCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG440.315570537187119No Hit
TCCCGGCTGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG430.30839847952377536No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT430.30839847952377536No Hit
TTCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG430.30839847952377536No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT420.30122642186043175No Hit
TCCCGGCTGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG420.30122642186043175No Hit
TCCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT410.29405436419708814No Hit
TCCCGGCTGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG400.2868823065337445No Hit
TTCCGGCCGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT400.2868823065337445No Hit
TTCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT400.2868823065337445No Hit
TTCCGGCCGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC400.2868823065337445No Hit
TTCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG390.2797102488704009No Hit
TTCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG390.2797102488704009No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT380.2725381912070573No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT380.2725381912070573No Hit
TCCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG370.2653661335437137No Hit
TTCCGGCCGACCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG370.2653661335437137No Hit
TCCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT370.2653661335437137No Hit
TCCCGGCCGATCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG370.2653661335437137No Hit
TCCCGGCTGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG350.25102201821702647No Hit
TCCCGGCTGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG350.25102201821702647No Hit
TCCCGGCCGATCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC340.24384996055368283No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT340.24384996055368283No Hit
TTCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG330.23667790289033924No Hit
TCCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.22950584522699563No Hit
TCCCGGCCGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT320.22950584522699563No Hit
TCCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG320.22950584522699563No Hit
TTCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG310.222333787563652No Hit
TCCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT310.222333787563652No Hit
TCCCGGCCGATCGTCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG300.21516172990030838No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT300.21516172990030838No Hit
TCCCGGCCGACCGTCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG300.21516172990030838No Hit
TCCCGGCTGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG300.21516172990030838No Hit
TTCCGGCCGATCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG270.19364555691027757No Hit
TCCCGGCCGACCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG270.19364555691027757No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC250.17930144158359035No Hit
TCCCGGCCGATCGCCTGATTGCCATGTCACTTTCGTTCAAGACGACGCCG230.1649573262569031No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC190.13626909560352865No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC190.13626909560352865No Hit
TCCCGGCCGACCGCCTGATCGCCATGTCACTTTCGTTCAAGACGACGCCG170.12192498027684141No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC160.11475292261349782No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC160.11475292261349782No Hit
TTCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGC150.10758086495015419No Hit
TCCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC150.10758086495015419No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC150.10758086495015419No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC140.10040880728681059No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGC140.10040880728681059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)