FastQCFastQC Report
Fri 25 Oct 2024
000000000-GM647_l01_n01_P_HSD_ATH13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GM647_l01_n01_P_HSD_ATH13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13378
Sequences flagged as poor quality0
Sequence length301
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGGCTGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1891.4127672297802363No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1821.3604425175661534No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1661.2408431753625355No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1641.2258932575870831No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1611.203468380923905No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1581.1810435042607266No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1571.1735685453730005No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1551.1586186275975483No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1551.1586186275975483No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1541.151143668709822No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1511.1287187920466437No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1501.1212438331589176No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1501.1212438331589176No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1481.1062939153834654No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1471.0988189564957391No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1421.0614441620571087No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1411.0539692031693826No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1381.0315443265062043No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1361.0165944087307521No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1341.0016444909552997No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1320.9866945731798474No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1310.9792196142921215No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1300.9717446554043953No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1280.9567947376289431No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1210.9044700254148602No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1150.8596202720885034No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1140.8521453132007775No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1130.8446703543130513No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1070.7998206009866946No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1060.7923456420989685No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAATGGCGTGATGAACGCAGTG1050.7848706832112423No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAATGGCGTGATGAACGCAGTG1050.7848706832112423No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG910.6802212587830767No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG840.6278965465689939No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG740.5531469576917327No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG730.5456719988040066No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG730.5456719988040066No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG720.5381970399162804No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG710.5307220810285543No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG700.5232471221408282No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG670.5008222454776499No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG640.47839736881447154No Hit
TCCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG620.4634474510390193No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG610.45597249215129315No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG610.45597249215129315No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG600.448497533263567No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG590.4410225743758409No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG590.4410225743758409No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG560.41859769771266253No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG560.41859769771266253No Hit
TCCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG530.39617282104948426No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG530.39617282104948426No Hit
TCCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG510.38122290327403197No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG500.3737479443863059No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG500.3737479443863059No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG500.3737479443863059No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG490.36627298549857973No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG490.36627298549857973No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG490.36627298549857973No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG490.36627298549857973No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG470.35132306772312755No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG470.35132306772312755No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAACGCGGTG440.32889819105994916No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAACGCGGTG430.3214232321722231No Hit
TTCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA310.23172372551950965No Hit
TTCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA290.2167738077440574No Hit
TCCCGGCTGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA290.2167738077440574No Hit
TTCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA270.20182388996860517No Hit
TCCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA270.20182388996860517No Hit
TCCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA260.19434893108087906No Hit
TCCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA260.19434893108087906No Hit
TTCCGGCTGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA260.19434893108087906No Hit
TTCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA260.19434893108087906No Hit
TTCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA250.18687397219315294No Hit
TTCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA250.18687397219315294No Hit
TCCCGGCTGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA240.17939901330542682No Hit
TCCCGGCTGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA230.1719240544177007No Hit
TTCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT230.1719240544177007No Hit
TCCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA230.1719240544177007No Hit
TTCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC230.1719240544177007No Hit
TTCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA220.16444909552997458No Hit
TTCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT220.16444909552997458No Hit
TTCCGGCTGACCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT220.16444909552997458No Hit
TTCCGGCCGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT210.15697413664224846No Hit
TTCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA210.15697413664224846No Hit
TTCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA200.14949917775452234No Hit
TTCCGGCTGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA200.14949917775452234No Hit
TTCCGGCCGATCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA200.14949917775452234No Hit
TTCCGGCCGATCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA200.14949917775452234No Hit
TCCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT190.14202421886679623No Hit
TCCCGGCCGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT190.14202421886679623No Hit
TTCCGGCCGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT180.1345492599790701No Hit
TCCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.1345492599790701No Hit
TTCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.1345492599790701No Hit
TCCCGGCTGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT180.1345492599790701No Hit
TCCCGGCTGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC180.1345492599790701No Hit
TCCCGGCTGATCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT180.1345492599790701No Hit
TCCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC180.1345492599790701No Hit
TTCCGGCTGATCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC170.12707430109134402No Hit
TTCCGGCCGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA170.12707430109134402No Hit
TCCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC170.12707430109134402No Hit
TCCCGGCCGATCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA170.12707430109134402No Hit
TTCCGGCTGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT170.12707430109134402No Hit
TTCCGGCCGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA170.12707430109134402No Hit
TCCCGGCCGATCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA170.12707430109134402No Hit
TCCCGGCCGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC160.11959934220361788No Hit
TCCCGGCCGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA160.11959934220361788No Hit
TTCCGGCCGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.11959934220361788No Hit
TTCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT160.11959934220361788No Hit
TTCCGGCTGACCGTCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC160.11959934220361788No Hit
TCCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT160.11959934220361788No Hit
TCCCGGCTGATCGCCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT160.11959934220361788No Hit
TCCCGGCCGACCGTCTGATCGCCAAGAAAACAAGACAGCTCAAAACCAGT160.11959934220361788No Hit
TTCCGGCTGATCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT160.11959934220361788No Hit
TCCCGGCCGACCGTCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA150.11212438331589175No Hit
TTCCGGCTGATCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC150.11212438331589175No Hit
TTCCGGCTGATCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC150.11212438331589175No Hit
TTCCGGCCGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT150.11212438331589175No Hit
TTCCGGCTGACCGCCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC150.11212438331589175No Hit
TTCCGGCCGACCGTCTGATTGCCATTTTACATGGTCTTGAAGCCCTCGAC150.11212438331589175No Hit
TTCCGGCTGACCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT150.11212438331589175No Hit
TTCCGGCTGACCGCCTGATCGCCATTTTACATGGTCTTGAAGCCCTCGAC140.10464942442816563No Hit
TCCCGGCTGACCGCCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA140.10464942442816563No Hit
TTCCGGCCGATCGCCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT140.10464942442816563No Hit
TCCCGGCTGACCGTCTGATTGCCAAGAAAACAAGACAGCTCAAAACCAGT140.10464942442816563No Hit
TTCCGGCTGACCGTCTGATCGCCAACGTCAACGGCGTGATGAATGCTGTA140.10464942442816563No Hit
TCCCGGCTGACCGCCTGATTGCCAACGTCAACGGCGTGATGAATGCTGTA140.10464942442816563No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)