FastQCFastQC Report
Tue 30 Jul 2024
000000000-GLVJ8_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GLVJ8_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641087
Sequences flagged as poor quality0
Sequence length8
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC84505851.49379648976562Illumina Single End PCR Primer 1 (100% over 8bp)
TATCCTCT675734.117575728770016No Hit
CTCTCTAT650213.9620690432621792ABI Solid3 Adapter B (100% over 8bp)
AAGGAGTA437452.665611268628659ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
ACTGCATA430852.625394022376632No Hit
AGAGTAGA421932.5710398047148018No Hit
TAGATCGC298921.8214756438872528No Hit
GTAAGGAG275621.6794965775732793No Hit
CTAAGCCT219301.3363094095559833No Hit
TGTAATGA107470.654870826470504No Hit
AGCTAGAG105740.6443290331347454No Hit
GTGAACCA105380.6421353651573621No Hit
AGGTTGCA95290.5806517265690363No Hit
ATTGACAT94190.5739488521936984RNA PCR Primer, Index 15 (100% over 8bp)
TACTAGGT90740.5529262007437753No Hit
CTGTGCTA87820.5351331160383331No Hit
CACGTCAG86730.5284911768845892No Hit
GTGCTGAA86280.5257490919128602No Hit
AGCCAACT85690.5221539138388154No Hit
TAAGATGG84670.5159385212362294No Hit
GGCCATAA77440.4718823560237818No Hit
TCTCTGGA77000.4692012062736467No Hit
TCACGGTT73760.44945819447719715No Hit
AGTCCTTC73440.44750826738618976No Hit
GACCGCCA66720.40655979847503515No Hit
TGGCCATG66350.40430519527605785No Hit
CGCGGTTA65800.400953758088389No Hit
TCCTTACG65740.40058814675882515No Hit
GAGAGATT58010.3534852204666785No Hit
CCTCAGAC57700.3515962285972651No Hit
GGCTACTC55530.3383732855113714No Hit
AGCATTGG55230.33654522886355204No Hit
ACGCGTGG55190.33630148797717613No Hit
GGAACTCC52780.3216160995730269RNA PCR Primer, Index 1 (100% over 8bp)
AGCACCAA50430.3072963224984416No Hit
TGTCGAAC50160.3056510715154041No Hit
CGGAAGAA48770.2971810757138409No Hit
TGAGGCAT48570.2959623712819613No Hit
ATGTCGTG47280.28810172769633785No Hit
ACGCACAA46380.2826175577528796No Hit
TCGGTTCA45120.27493971983203813No Hit
AGACGGAA45000.2742084971729104TruSeq Adapter, Index 14 (100% over 8bp)
TGCGTGAA44030.26829778067829435No Hit
CTTGAGGA43280.2637276390587458No Hit
AGGAACTG43130.2628136107348361No Hit
GTGATAGG42550.25927936788238526No Hit
CCGTAACA41190.25099217774560395No Hit
GACAATGC40740.24825009277387486No Hit
TGTTAGCC40600.24739699967155915No Hit
CTGACTTC40020.24386275681910832No Hit
AGTTCAGG39370.23990196741549963No Hit
TCCAACAG38580.23508808490957517No Hit
TGTAGGTG38380.23386938047769557No Hit
GCAGAGAA38240.23301628737537988No Hit
GCACTAAC37540.22875082186380125No Hit
TTGCCTGA37540.22875082186380125No Hit
GTTCGAGA37420.22801959920467346No Hit
AACTCTCG36970.22527751423294437TruSeq Adapter, Index 13 (100% over 8bp)
ATGGATGC36220.2207073726133959No Hit
ATACGTCC36210.2206464373918019No Hit
TTACCGAC36100.21997614995426812No Hit
GTCACAAC35670.21735593542572695No Hit
ATAGCCTC35670.21735593542572695No Hit
CGGTTAAC34890.21260298814139655No Hit
TCGTGGAA34860.2124201824766146No Hit
CTAGGAAC34810.21211550636864468No Hit
AAGGTACC32820.19998939727144263No Hit
CACTGAGA31240.1903616322595938No Hit
CATAGACC29650.18067293202615095No Hit
TTGTGAGC27890.16994833302561047No Hit
GTTAGCTC27200.16574380273562583No Hit
TATTTCCC20060.12223605451752405No Hit
TCGAAGTC19860.12101735008564446No Hit
GTAATGAG19370.11803152422753943No Hit
TTAAGGAG18510.11279109517045714No Hit
AGCGTAGA18410.11218174295451736No Hit
CAATGGTC17390.10596635035193136No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)