FastQCFastQC Report
Tue 30 Jul 2024
000000000-GLVJ8_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GLVJ8_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641087
Sequences flagged as poor quality0
Sequence length8
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGAGTT1544399.41077468775269TruSeq Adapter, Index 13 (100% over 8bp)
ACGCTACT1107936.751196006061836No Hit
ACTCACTG696594.244686601015059No Hit
CCTAAGAC276561.6852244884031131No Hit
ACTGAGCG274301.6714531283228737No Hit
CGATCAGT264971.6146005665756902No Hit
AAGAGGCA198511.2096250838620988No Hit
GTAGAGGA168291.0254788442050908No Hit
ATGCGCAG143620.875151652532742No Hit
TAGCGCTC140870.8583944665943974No Hit
AGGCAGAA122560.74682207585582No Hit
CGAGGCTG120090.7317710761221069No Hit
TTTTTTTT118140.7198887079112808No Hit
GCTACGCT108220.6594409680900525No Hit
CTCTCTAC107070.6524334176067448No Hit
CGTACTAG97270.5927169004446443No Hit
TAGGCATG81480.4965001855477497No Hit
TAAGGCGA77410.47169955035899985No Hit
ACTCGCTA76190.46426545332453434No Hit
GGACTCCT76130.4638998419949704No Hit
ATCTCAGG73840.44994567624994897No Hit
TACGCTGC72710.4430599962098292No Hit
CAGAGAGG70580.43008079401031146No Hit
TCCTGAGC66590.40576764059431336No Hit
CGGAGCCT63510.3869995923433675No Hit
CGATCATT60620.3693893133027073No Hit
GGAGCTAC51630.3146085490897192No Hit
GCTCATGA50990.3107086949077045No Hit
TTATTTTT50200.30589481240178007No Hit
GCGTAGTA48460.2952920838444275No Hit
TTTTTTTG46310.2821910112017218No Hit
TAAAAAAA42560.25934030310397926No Hit
TTAGAGGA40800.24861570410343875No Hit
ACTACGAC39430.2402675787450635No Hit
TTTTTTTA37500.22850708097742534No Hit
TCGCTACT37290.22722744132395176No Hit
GACATAGT33970.2069969477547504No Hit
GTAGAGTA33680.20522982632852493No Hit
TAAAATAA33160.20206119480563797No Hit
CTCTCTTT32630.19883162806115703No Hit
ACTACGCT32550.19834414628840516No Hit
TGAGAGTT30930.1884726403901804No Hit
TTATATTT29960.18256192389556433No Hit
ATAGAGGA29460.17951516281586533No Hit
TTCTTTTT28700.17488408597472285No Hit
TCTACGCT27240.16598754362200177No Hit
TTATTTTA26750.16300171776389674No Hit
AAGATGCA26360.16062524412173151No Hit
TTCTCTTT25440.15501920373508535No Hit
TAAATAAA24600.149900645121191No Hit
TAGTCTCC24320.14819445891655958No Hit
TATAGCGA23620.14392899340498094No Hit
TATTTTTT23510.1432587059674472No Hit
TTTTATTT23390.14252748330831944No Hit
TTATATTA23020.14027288010934216No Hit
TTTTTATT22660.13807921213195887No Hit
TGACTCCT22340.1361292850409515No Hit
TAGCTCTC22100.134666839722696No Hit
GTCTGCTA22080.13454496927950804No Hit
CGAGCGAC22030.13424029317153813No Hit
ATGCTCAG22020.13417935794994415No Hit
AGACTCCT21920.13357000573400435No Hit
TTATTTTG21720.13235130130212475No Hit
GTCTATGA21250.12948734588720767No Hit
TCTCACTG20230.12327195328462172No Hit
TGAGTACG20230.12327195328462172No Hit
CGAGAGTG20180.12296727717665182No Hit
TTATTATT19570.11925022865941902No Hit
TTTATTTT19560.11918929343782506No Hit
TAAAATTA19470.11864087644347923No Hit
TTTTTTAT19360.11797058900594545No Hit
ATCTCATG18930.11535037447740429No Hit
TAAATTAA18910.11522850403421635No Hit
TACGCTTC18840.11480195748305849No Hit
CGAGTCTG18830.1147410222614645No Hit
TTTTATTA18640.11358325305117889No Hit
TTTTATTG18570.11315670650002103No Hit
TAATAAAA18570.11315670650002103No Hit
TAATATAA18360.11187706684654744No Hit
TTATTTAA18340.11175519640335947No Hit
TTTTTTAA18130.11047555674988589No Hit
TAATTTTT18070.11010994542032201No Hit
TTATATAA17830.1086475001020665No Hit
AAAAAAAA17600.10724599000540495No Hit
TAAAAATA17290.10535699813599157No Hit
TTAAATAA17170.1046257754768638No Hit
TTATTTAT17150.10450390503367585No Hit
CTGCGTAG16510.10060405085166113No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)