FastQCFastQC Report
Wed 15 May 2024
000000000-GLMFC_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-GLMFC_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3753407
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC72472219.308377695251274Illumina Single End PCR Primer 1 (100% over 8bp)
TATCCTCT1376223.6665887818720435No Hit
CTCTCTAT1216613.2413484602122815ABI Solid3 Adapter B (100% over 8bp)
GCAGAGAA1176103.1334198502853545No Hit
ACTGCATA1153243.0725151842046436No Hit
ACGCACAA1129223.0085199926360238No Hit
AGCATTGG1111782.9620555404729623No Hit
TAGATCGC1111712.9618690432452435No Hit
TTGCCTGA1102112.9362922805866774No Hit
GTAAGGAG1100482.931949559426942No Hit
TTACCGAC1055852.813044255525713No Hit
CCGTAACA1050652.799190175752323No Hit
CTAGGAAC1028942.7413493926984205No Hit
GTGATAGG986542.6283853576230873No Hit
AGAGTAGA921492.4550761481502006No Hit
CTTGAGGA898222.393079141164281No Hit
GTTCGAGA891402.374908982692258No Hit
CTGACTTC884092.355433343626204No Hit
GTCACAAC856302.2813939442218762No Hit
AACTCTCG844322.249476275820874TruSeq Adapter, Index 13 (100% over 8bp)
AAGGAGTA811992.163341199075933ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
CTAAGCCT785182.091912760859667No Hit
TCGTGGAA747691.9920301741857465No Hit
TCCAACAG643531.714522299340306No Hit
GTTAGCTC631761.6831641226224598No Hit
TGTCGAAC595901.5876242571082753No Hit
CACTGAGA592481.578512535411161No Hit
ATGGATGC584021.5559730133182998No Hit
AAGGTACC562331.4981855151866026No Hit
AGACGGAA560331.492857022966068TruSeq Adapter, Index 14 (100% over 8bp)
TTGTGAGC484351.2904276035079596No Hit
TCGAAGTC214350.5710811537357926No Hit
CAATGGTC205750.5481686371874939No Hit
CGGAACAA58040.15463284423991322No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)